|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||40 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , 293T(starvation16 hours) , 293T(starvation16 hours,10uM forskolin for 20 mins)|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||vasodilator-stimulated phosphoprotein|
|Product Description||Rabbit Polyclonal antibody to VASP (vasodilator-stimulated phosphoprotein)|
|Background||Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]|
|Synonyms||vasodilator-stimulated phosphoprotein Antibody|
|Cellular Localization||Cytoplasm , cytoskeleton , Cell junction , focal adhesion , Cell projection , lamellipodium membrane , filopodium membrane |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VASP. The exact sequence is proprietary.|