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1 .GTX113099 VCP antibody [N3C2], Internal

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VCP antibody [N3C2], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
Cat No: GTX113099
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
  • Anti-VCP antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113099
  • Anti-VCP antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX113099
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  • Application Information: VCP antibody [N3C2], Internal

Catalog Number GTX113099
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Product Name VCP antibody [N3C2], Internal
See all VCP products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:20000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size89 kDa (note)
Positive Controls293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , Rat brain
Form SuppliedLiquid
Concentration0.2 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: VCP antibody [N3C2], Internal

Full Namevalosin containing protein
BackgroundThe protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
SynonymsIBMPFD antibody, MGC131997 antibody, MGC148092 antibody, MGC8560 antibody, TERA antibody, p97 antibody, VCP antibody, yeast Cdc48p homolog antibody, 15S Mg(2+)-ATPase p97 subunit antibody, TER ATPase antibody, transitional endoplasmic reticulum ATPase antibody, valosin-containing protein antibody, valosin containing protein antibody
Cellular LocalizationCytoplasm , cytosol , Nucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human VCP. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Zebrafish, Xenopus laevis, Pig, Chicken, Chimpanzee, Bovine
Predict Reactivity NoteZebrafish (98%), Xenopus laevis (98%), Pig (99%), Chicken (100%), Chimpanzee (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: VCP antibody [N3C2], Internal

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Cell cycle > Protein ubiquitination
  • Cell Biology > Ubiquitination and Protein degradation > Ubiquitination
  • Signal Transduction > Protein ubiquitination > Ubiquitination
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VCP antibody [N3C2], Internal validated data
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
    • GTX113099 WB Image
      VCP antibody detects VCP protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GTX113099) diluted by 1:1000.
      Top
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
    • GTX113099 WB Image
      VCP antibody [N3C2], Internal detects VCP protein by western blot analysis.
      A. 50 µg Rat brain lysate/extract
      7.5% SDS-PAGE
      VCP antibody [N3C2], Internal (GTX113099) dilution: 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
    • GTX113099 WB Image
      Sample (30 µg of whole cell lysate)
      A: NIH-3T3
      B: JC
      C: BCL-1
      7.5% SDS PAGE
      GTX113099 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-VCP antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113099
    • GTX113099 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using VCP(GTX113099) antibody at 1:500 dilution.
      Top
  • Anti-VCP antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX113099
    • GTX113099 IHC-P Image
      VCP antibody [N3C2], Internal detects VCP protein at nucleus on human gastric cancer by immunohistochemical analysis.
      Sample: Paraffin-embedded gastric cancer.
      VCP antibody [N3C2], Internal (GTX113099) dilution: 1:500.
      Top
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
    • GTX113099 WB Image
      VCP antibody detects VCP protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody (GTX113099) diluted by 1:1000.
      Top
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
    • GTX113099 WB Image
      VCP antibody [N3C2], Internal detects VCP protein by western blot analysis.
      A. 50 µg Rat brain lysate/extract
      7.5% SDS-PAGE
      VCP antibody [N3C2], Internal (GTX113099) dilution: 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-VCP antibody [N3C2], Internal used in Western Blot (WB). GTX113099
    • GTX113099 WB Image
      Sample (30 µg of whole cell lysate)
      A: NIH-3T3
      B: JC
      C: BCL-1
      7.5% SDS PAGE
      GTX113099 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-VCP antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113099
    • GTX113099 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using VCP(GTX113099) antibody at 1:500 dilution.
      Top
  • Anti-VCP antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX113099
    • GTX113099 IHC-P Image
      VCP antibody [N3C2], Internal detects VCP protein at nucleus on human gastric cancer by immunohistochemical analysis.
      Sample: Paraffin-embedded gastric cancer.
      VCP antibody [N3C2], Internal (GTX113099) dilution: 1:500.
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensVCP7415Q2TAI5, P55072167320, 601023NM_007126NP_00905789.33

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusVcp269523Q01853NM_009503NP_03352989.33
Rattus norvegicusVcp116643P46462NM_053864NP_44631689.36
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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