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  • VEGF antibody [4G3]

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1 .GTX83425 VEGF antibody [4G3]

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VEGF antibody [4G3]


  • Host / ClonalityMouse Monoclonal
  • Clone Name4G3
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Dog
Cat No: GTX83425
Package Price Qty
100 μlUSD 379.00
Add to cart
 
  • Anti-VEGF antibody [4G3] used in Western Blot (WB). GTX83425
  • Anti-VEGF antibody [4G3] used in Western Blot (WB). GTX83425
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
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  • Datasheet

  • Application Information: VEGF antibody [4G3]

Catalog Number GTX83425
☆☆☆☆☆
( 0 )
Publication ( 0 )
Product Name VEGF antibody [4G3]
See all VEGF products
ApplicationsIHC-P, WB
Application NoteRecommended Starting Dilutions:

WB 1:1000
IHC 1:50
Optimal working dilutions should be determined experimentally by the end user.

Form SuppliedLiquid
PurificationProtein A/G purified

  • Specifications: VEGF antibody [4G3]

Full Namevascular endothelial growth factor A
BackgroundGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
SynonymsVEGFA, VPF, MGC70609
SpecificityGTX83425 is specific for amino acids 27-233 of human VEGF
HostMouse
ClonalityMonoclonal
Clone Name4G3
IsotypeIgG2b
ImmunogenRecombinant protein expressed in E.coli corresponding to amino acids 27-233 of human VEGF
Antigen SpeciesHuman
Species ReactivityHuman, Dog
ConjugationUnconjugated

  • Storage Conditions: VEGF antibody [4G3]

Storage BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Storage InstructionFor short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Angiogenesis
  • Cancer > Apoptosis > Anti-apoptosis
  • Cancer > Apoptosis > Metastasis and Invasion > Invasion
  • Cancer > Apoptosis > Metastasis and Invasion > Metastasis > Lymphatic
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VEGF antibody [4G3] validated data
  • Anti-VEGF antibody [4G3] used in Western Blot (WB). GTX83425
    • GTX83425 WB Image
      HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF (GTX83425).
      Top
  • Anti-VEGF antibody [4G3] used in Western Blot (WB). GTX83425
    • GTX83425 WB Image
      Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VEGF monoclonal antibody.
      Top
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
    • GTX83425 IHC-P Image
      Immunohistochemical staining of paraffin-embedded human breast tissue using anti-VEGF mouse monoclonal antibody. (GTX83425)
      Top
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
    • GTX83425 IHC-P Image
      Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-VEGF mouse monoclonal antibody. (GTX83425)
      Top
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
    • GTX83425 IHC-P Image
      Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-VEGF mouse monoclonal antibody. (GTX83425)
      Top
  • Anti-VEGF antibody [4G3] used in Western Blot (WB). GTX83425
    • GTX83425 WB Image
      HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF (GTX83425).
      Top
  • Anti-VEGF antibody [4G3] used in Western Blot (WB). GTX83425
    • GTX83425 WB Image
      Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VEGF monoclonal antibody.
      Top
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
    • GTX83425 IHC-P Image
      Immunohistochemical staining of paraffin-embedded human breast tissue using anti-VEGF mouse monoclonal antibody. (GTX83425)
      Top
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
    • GTX83425 IHC-P Image
      Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-VEGF mouse monoclonal antibody. (GTX83425)
      Top
  • Anti-VEGF antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83425
    • GTX83425 IHC-P Image
      Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-VEGF mouse monoclonal antibody. (GTX83425)
      Top
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