VPS25 antibody, Internal
|Applications||IHC, IHC-P, WB|
|Application Note||Approx 20-22kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 µg/ml. In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Recommended concentration, 10µg/ml. |
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX89428-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||vacuolar protein sorting 25 homolog (S. cerevisiae)|
|Product Description||Goat polyclonal antibody to VPS25|
|Background||Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. |
|Synonyms||MGC10540, FAP20, vacuolar protein sorting25 homolog (S. cerevisiae), VPS25, HGNC:28122, vacuolar protein sorting 25 (yeast), EAP20, vacuolar protein sorting25 (yeast), vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein sorting25, DERP9, vacuolar protein sorting 25|
|Cellular Localization||Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6. |
|Specificity||This antibody is specific for VPS25.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Dog|