VPS28 antibody, C-term
|Applications||IHC, IHC-P, WB|
|Application Note||Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3µg/ml. In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration, 10µg/ml. This antibody is expected to recognize isoform 1 (NP_057292.1) only.|
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX10133-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||vacuolar protein sorting 28 homolog (S. cerevisiae)|
|Product Description||Goat polyclonal antibody to VPS28|
|Background||This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.|
|Synonyms||vacuolar protein sorting28 homolog (S. cerevisiae), vacuolar protein sorting28, vacuolar protein sorting 28 (yeast), vacuolar protein sorting 28 homolog (S. cerevisiae), MGC60323, vacuolar protein sorting 28, yeast class E protein Vps28p homolog, VPS28, vacuolar protein sorting28 (yeast)|
|Specificity||This antibody is specific for VPS28.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Dog, Pig|