|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||54 kDa (note)
|Observed Size||56 kDa.
The observed M.W. is based on the following publication. PMID:23185433 and 24926698
|Positive Controls||293T , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , NIH-3T3 , MDA-MB-231 , PC-12 , Rat2 , A549*|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Product Description||Rabbit Polyclonal antibody to Vimentin (vimentin)|
|Background||Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]|
|Synonyms||FLJ36605 antibody, VIM antibody, vimentin antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Dog, Chicken, Chimpanzee, Bovine, Guinea pig|
|Predict Reactivity Note||Xenopus laevis (84%), Dog (98%), Chicken (92%), Chimpanzee (99%), Bovine (98%), Guinea pig (97%)|