Vitronectin antibody

Host / ClonalityRabbit Polyclonal
ApplicationsELISA, IHC-P, WB
Species reactivityHuman, Mouse
( 1 )

Cat No:

GTX103475

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Protocol & MSDS

Application Information: Vitronectin antibody

Catalog Number

GTX103475

Product Name

Vitronectin antibody

See all Vitronectin products

Applications

ELISA, IHC-P, WB

Application Note

	Suggested dilution	Reference
ELISA	Assay-dependent dilution	Y
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Western Blot (WB)	1:500-1:3000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

54 kDa (note)

Positive Controls

HepG2 conditioned medium , mouse brain

Form Supplied

Liquid

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Vitronectin antibody

Full Name	vitronectin
Background	The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq]
Synonyms	V75 antibody, VN antibody, VNT antibody, VTN antibody, complement S-protein antibody, S-protein antibody, vitronectin antibody, epibolin antibody, serum-spreading factor antibody, serum spreading factor antibody, somatomedin B antibody
Cellular Localization	Secreted , extracellular space
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human Vitronectin. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse
Conjugation	Unconjugated

Storage Conditions: Vitronectin antibody

Storage Buffer	0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Type of cancer > Thyroid Cell Biology > Cell adhesion > Cell-substrate adhesion Cell Biology > Cell adhesion > Regulation > Positive regulation Signal Transduction > ECM/Cytoskeleton > Cell Adhesion More Hide

Vitronectin antibody validated data

GTX103475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vitronectin(GTX103475) antibody at 1:500 dilution.
Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

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GTX103475 WB Image

HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX103475 WB Image

Vitronectin antibody detects Vitronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX103475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vitronectin(GTX103475) antibody at 1:500 dilution.
Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX103475 WB Image

HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX103475 WB Image

Vitronectin antibody detects Vitronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (GTX103475) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

Reference for Vitronectin antibody

Application References	Application	Reactivity
Lu CH et al. Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients. Arch Biochem Biophys 529 (2):146-56 (2013).	ELISA	Human

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	VTN	7448	P04004	193190	NM_000638	NP_000629	54.32

*MSDS

MSDS-Glycerol.pdf

MSDS-Thimerosal.pdf

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Vitronectin antibody

Vitronectin antibody

Application Information: Vitronectin antibody

Specifications: Vitronectin antibody

Storage Conditions: Vitronectin antibody