WBSCR22 antibody

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, IHC-P, IP, WB
Species reactivityHuman, Mouse
★★★☆☆ ( 1 ) ( 2 )

Cat No:

GTX105840

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Star Review (1)
Reference (2)
Bio-info
Protocol & MSDS

Application Information: WBSCR22 antibody

Catalog Number

GTX105840

Product Name

WBSCR22 antibody

See all WBSCR22 products

Applications

ICC/IF, IHC-P, IP, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:1000*	Y
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Immunoprecipitation	1:500-1:1000*
Western Blot (WB)	1:500-1:3000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

32 kDa (note) IsoForm

(2)

Positive Controls

HeLa , HCT116 , *A375M

Form Supplied

Liquid

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: WBSCR22 antibody

Full Name	Williams Beuren syndrome chromosome region 22
Background	This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq]
Synonyms	FLJ44236 antibody, HASJ4442 antibody, HUSSY-3 antibody, MGC19709 antibody, MGC2022 antibody, MGC5140 antibody, PP3381 antibody, WBMT antibody, WBSCR22 antibody, Williams-Beuren candidate region putative methyltransferase antibody, Williams-Beuren syndrome chromosomal region 22 protein antibody, uncharacterized methyltransferase WBSCR22 antibody, Williams Beuren syndrome chromosome region 22 antibody
Cellular Localization	Nucleus
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human WBSCR22. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Rhesus Monkey, Bovine
Predict Reactivity Note	Rhesus Monkey (99%), Bovine (83%)
Conjugation	Unconjugated

Storage Conditions: WBSCR22 antibody

Storage Buffer	0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

WBSCR22 antibody validated data

GTX105840 IP Image

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 μg of WBSCR22 antibody (GTX105840) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (GTX105840) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

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GTX105840 IHC-P Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WBSCR22 antibody (GTX105840) diluted at 1:500.

Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

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GTX105840 ICC/IF Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WBSCR22 protein stained by WBSCR22 antibody (GTX105840) diluted at 1:200.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

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GTX105840 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (GTX105840) antibody at 1:100 dilution.
Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

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GTX105840 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HCT116
12% SDS PAGE
GTX105840 diluted at 1:1000

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GTX105840 IP Image

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 μg of WBSCR22 antibody (GTX105840) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (GTX105840) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

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GTX105840 IHC-P Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WBSCR22 antibody (GTX105840) diluted at 1:500.

Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX105840 ICC/IF Image

WBSCR22 antibody detects WBSCR22 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WBSCR22 protein stained by WBSCR22 antibody (GTX105840) diluted at 1:200.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

Top

GTX105840 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (GTX105840) antibody at 1:100 dilution.
Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX105840 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HCT116
12% SDS PAGE
GTX105840 diluted at 1:1000

Top

StarReview
WBSCR22 antibody
Rating : ★★★☆☆ ( Average 3.0 based on 1 users reviews)
Western Blot (WB)(1) :★★★☆☆

Anonymous StarReview submitted on 12 Mar 2014
Score : ★★★☆☆
Application Tested : WB
Sample Species : Human
Sample : A375M cells
Amount used(ug/cells) : 26ug
Blocking conditions
Blocking Buffer : 1% skim milk /TBST
Incubation Time & Temperature : 25 (℃)/1hr
Primary antibody
Dilution : 1:1000( conc. of dilution )
Incubation Time & Temperature : 25 (℃)/2hr

Reference for WBSCR22 antibody

Application References	Application	Reactivity
Zhao H et al. Natural killer cells inhibit oxaliplatin-resistant colorectal cancer by repressing WBSCR22 via upregulating microRNA-146b-5p. Am J Cancer Res 8 (5):824-834 (2018).	WB	Human
Nakazawa Y et al. The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis. Cancer Res 71 (3):1146-55 (2011).	ICC/IF	Human

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	WBSCR22	114049	O43709, A8K501		NM_001202560	NP_001189489	33.85
Homo sapiens	WBSCR22	114049	O43709, A8K501		NM_017528	NP_059998	31.88

*MSDS

MSDS-Glycerol.pdf

MSDS-Thimerosal.pdf

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