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1 .GTX105840 WBSCR22 antibody

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WBSCR22 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Mouse
  • ★★★☆☆   (  1  ) Publication  ( 1 )
Cat No: GTX105840
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-WBSCR22 antibody used in Immunoprecipitation. GTX105840
  • Anti-WBSCR22 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105840
  • Anti-WBSCR22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105840
  • Anti-WBSCR22 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105840
  • Anti-WBSCR22 antibody used in Western Blot (WB). GTX105840
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  • Datasheet
  • Star Review (1)
    Reference (1)
  • Bio-info
  • Protocol & MSDS
  • Application Information: WBSCR22 antibody

Catalog Number GTX105840
★★★☆☆
( 1 )
Publication ( 1 )
Product Name WBSCR22 antibody
See all WBSCR22 products
ApplicationsICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000* Y
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:500-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size32 kDa (note) IsoForm (2)
Positive ControlsHeLa , HCT116 , *A375M
Form SuppliedLiquid
Concentration0.42 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: WBSCR22 antibody

Full NameWilliams Beuren syndrome chromosome region 22
BackgroundThis gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq]
SynonymsFLJ44236 antibody, HASJ4442 antibody, HUSSY-3 antibody, MGC19709 antibody, MGC2022 antibody, MGC5140 antibody, PP3381 antibody, WBMT antibody, WBSCR22 antibody, Williams-Beuren candidate region putative methyltransferase antibody, Williams-Beuren syndrome chromosomal region 22 protein antibody, uncharacterized methyltransferase WBSCR22 antibody, Williams Beuren syndrome chromosome region 22 antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human WBSCR22. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rhesus Monkey, Bovine
Predict Reactivity NoteRhesus Monkey (99%), Bovine (83%)
ConjugationUnconjugated

  • Storage Conditions: WBSCR22 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
WBSCR22 antibody validated data
  • Anti-WBSCR22 antibody used in Immunoprecipitation. GTX105840
    • GTX105840 IP Image
      WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 μg of WBSCR22 antibody (GTX105840) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (GTX105840) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-WBSCR22 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105840
    • GTX105840 IHC-P Image
      WBSCR22 antibody detects WBSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      WBSCR22 antibody (GTX105840) diluted at 1:500.
      Top
  • Anti-WBSCR22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105840
    • GTX105840 ICC/IF Image
      WBSCR22 antibody detects WBSCR22 protein at cytoplasm and nucleus by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: WBSCR22 protein stained by WBSCR22 antibody (GTX105840) diluted at 1:200.
      Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-WBSCR22 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105840
    • GTX105840 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (GTX105840) antibody at 1:100 dilution.
      Top
  • Anti-WBSCR22 antibody used in Western Blot (WB). GTX105840
    • GTX105840 WB Image
      Sample (30 ug of whole cell lysate)
      A: HeLa
      B: HCT116
      12% SDS PAGE
      GTX105840 diluted at 1:1000
      Top
  • Anti-WBSCR22 antibody used in Immunoprecipitation. GTX105840
    • GTX105840 IP Image
      WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 μg of WBSCR22 antibody (GTX105840) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (GTX105840) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-WBSCR22 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105840
    • GTX105840 IHC-P Image
      WBSCR22 antibody detects WBSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      WBSCR22 antibody (GTX105840) diluted at 1:500.
      Top
  • Anti-WBSCR22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105840
    • GTX105840 ICC/IF Image
      WBSCR22 antibody detects WBSCR22 protein at cytoplasm and nucleus by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: WBSCR22 protein stained by WBSCR22 antibody (GTX105840) diluted at 1:200.
      Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-WBSCR22 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX105840
    • GTX105840 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (GTX105840) antibody at 1:100 dilution.
      Top
  • Anti-WBSCR22 antibody used in Western Blot (WB). GTX105840
    • GTX105840 WB Image
      Sample (30 ug of whole cell lysate)
      A: HeLa
      B: HCT116
      12% SDS PAGE
      GTX105840 diluted at 1:1000
      Top
  • StarReview
  • WBSCR22 antibody

  • Rating : ★★★☆☆ ( Average 3.0 based on 1 users reviews)
  • Western Blot (WB)(1) :★★★☆☆
  • Anonymous StarReview submitted on 12 Mar 2014
  • Score : ★★★☆☆
  • Application Tested : WB
  • Sample Species : Human
  • Sample : A375M cells
  • Amount used(ug/cells) : 26ug
  • Blocking conditions
  • Blocking Buffer : 1% skim milk /TBST
  • Incubation Time & Temperature : 25 (℃)/1hr
  • Primary antibody
  • Dilution : 1:1000( conc. of dilution )
  • Incubation Time & Temperature : 25 (℃)/2hr
Back to Top
  • Reference for WBSCR22 antibody
Application References Application Reactivity
Nakazawa Y et al. The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis. Cancer Res 71 (3):1146-55 (2011). ICC/IF Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensWBSCR22114049O43709, A8K501NM_001202560NP_00118948933.85
Homo sapiensWBSCR22114049O43709, A8K501NM_017528NP_05999831.88
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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