| Catalog Number | GTX01502 |
| Product Name | WGA Lectin (FITC) |
| Full Name | Wheat Germ agglutinin, Triticum vulgaris |
| Synonyms | | Wheat Germ agglutinin, Triticum vulgaris | Wheat germ agglutinin lectin | Wheat germ agglutinin | Triticum vulgaris | GlcNAc lectin | WGA | WGA Lectin | Triticum vulgaris lectin | |
| Product Description | Triticum vulgaris lectin against N-acetyl glucosamine (FITC) |
| Background | Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction. Inhibiting/Eluting sugars: 500 mM GlcNAc, dimer, trimmer or 100 mM acetic acid.
Carbohydrate-Binding Specificity of WGA: Pentaacetylchitopentaose >Tetraacetylchitotetraose > triacetylchitotriose > Diacetylchitobiose > GlcNAc
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| Specificity | N-acetyl glucosamine (GlcNAc) |
| Applications | ICC/IF. The usefulness of this product in other applications has not been determined. |
| Application Note | Immunofluorescence 1:250-1:1000. The optimum dilution should be determined by the individual lab. |
| Target | N-acetyl glucosamine (GlcNAc) |
| Conjugation | FITC |
| Conjuation Note | Affinity purified WGA is conjugated with Fluorescein-5- isothiocyanate (FITC-isomer 1) and purified on Gel filtration column; A max. = 490 nm, E max =520 nm, Ratio of A490/A280= 1.0-1.4 |
| Form Supplied | Liquid |
| Concentration | 5 mg/ml |
| Storage Buffer | 10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide |
| Storage Instruction | Store at 2-8°C. |
| Notes | For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. |