Knockout/Knockdown Validation
Knockout/Knockdown Validation:
GeneTex Incorporates Negative Controls into the Quality Assurance Process.

Antibodies are essential tools for both basic and clinical research. However, there has been growing distrust of commercially available antibodies with regard to specificity and performance variability. As a result, the research community and funding agencies have demanded that antibody suppliers increase their efforts to more thoroughly validate these reagents. One approach to address this is through knockout or knockdown of the target protein by CRISPR- or RNAi-mediated technologies.


Learn more about the CRISPR/Cas9 technology
Learn more about the knockdown technology


In support of this goal, GeneTex is conducting knockout/knockdown-based validation as a fundamental component of its antibody quality assurance process. To date, we have evaluated over four hundred of our most popular products covering multiple research topics, such as apoptosis, metabolism, epithelial–mesenchymal transition, and neurodegenerative diseases. We will continue to expand these efforts in the future. Antibodies that have passed the KO/KD test will be labeled with the KO/KD validated icon.


Knockout/Knockdown Validation GeneTex

 


Highlighted Products
 

Apoptosis

p53 antibody [D01]   PARP antibody [N2C1], Internal   Caspase 3 antibody   FOXO3A antibody [C3], C-term

p53 antibody [D01] (GTX70214)

  PARP antibody [N2C1], Internal (GTX112864)   Caspase 3 antibody (GTX110543)   FOXO3A antibody [C3], C-term (GTX100277)

 

Metabolism

IDH1 antibody   IDH2 antibody [GT673]   Citrate synthetase antibody [N2C3]   Glutamine synthetase antibody

IDH1 antibody (GTX105179)

  IDH2 antibody [GT673] (GTX628487)   Citrate synthetase antibody [N2C3] (GTX110624)   Glutamine synthetase antibody (GTX109121)

 

Epithelial–mesenchymal transition

E-Cadherin antibody   Vimentin antibody [GT812]   αSMA antibody   STAT3 antibody [C3], C-term

E-Cadherin antibody (GTX100443)

  Vimentin antibody [GT812] (GTX629744)   αSMA antibody (GTX100034)   STAT3 antibody [C3], C-term (GTX104616)

 

Neurodegenerative diseases

SOD1 antibody   Huntingtin antibody   HMGB1 antibody   GSK3 beta antibody [C1C3]

SOD1 antibody (GTX100554)

  Huntingtin antibody (GTX132433)   HMGB1 antibody (GTX101277)   GSK3 beta antibody [C1C3] (GTX111192)

 


Share your KO/KD data with us!

If you want to share your knockout/knockdown validation data using GeneTex antibodies with us, please feel free to send us an email or contact us for further information. With your posted results, you will qualify for the GeneTex Rewards Program.

 


GeneTex cooperates with EdiGene to offer CRISPR knockout-validated antibodies

GeneTex is working with the genome editing company EdiGene to validate its antibodies through the integration of EdiGene's innovative CRISPR-based technology that produces gene-specific knockout (KO) cell lines. Lysates from these cell lines, conveniently generated in commonly used cells like HeLa and HEK293T, are used for western blot assays of GeneTex's antibody reagents. The goal of this combined effort is to assure researchers of the specificity of GeneTex's antibodies and the broad utility of KO cell lines from