The bitter/sweet (and umami) story
Taruno et al have published a fascinating study in Nature identifying CALHM1 as a voltage-gated ion channel essential for ATP (as a neurotransmitter) release in taste buds. The authors found that CALHM1 knockout mice demonstrated greatly diminished perception of sweet, bitter, and umami tastes, while there was little change in the perception of sour and salty tastes. Thus, this paper frames CALHM1 as a pivotal component of the signal chain in taste bud type II receptor cells and opens the door to exciting speculation that CALHM isoforms may play roles in ATP release in other physiological processes.
GeneTex is proud to note that our DOPA Decarboxylase antibody (GTX30448) (AADC) was used in this study for tissue section immunofluorescence assays to examine taste bud morphology (which appeared normal) in the CALHM1-/- mice. This antibody was validated initially in rat samples, but has now been shown in multiple studies to also detect the mouse protein. It has been tested in ICC/IF, IHC-Fr, and western blot applications and has been cited in numerous publications (please see PMID: 23010799, PMID: 21242252, and PMID: 16611813).
If you want to learn more about this antibody, please see the datasheet at the GeneTex website.