Acylglycerol kinase antibody

Name: Acylglycerol kinase antibody
Brand: GeneTex
SKU: GTX107413
Price: 399.00 USD

Anti-Acylglycerol kinase antibody used in Western Blot (WB). GTX107413

Anti-Acylglycerol kinase antibody used in IHC (Paraffin sections) (IHC-P). GTX107413

Anti-Acylglycerol kinase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107413

Cat. No. GTX107413

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Acylglycerol kinase products

View product citations for antibody GTX107413 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

mouse heart , A431 , H1299

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Acylglycerol kinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949602

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acylglycerol kinase , CATC5 , CTRCT38 , MTDPS10 , MULK

Cellular Localization

Mitochondrion membrane

Database

Gene ID: 55750 AGK
UniProt: Q53H12 AGK

Research Area

DATA IMAGES

GTX107413 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody (GTX107413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107413 IHC-P Image

Acylglycerol kinase antibody detects Acylglycerol kinase protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Acylglycerol kinase antibody (GTX107413) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107413 IHC-P Image

Acylglycerol kinase antibody detects Acylglycerol kinase protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Acylglycerol kinase antibody (GTX107413) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107413 IHC-P Image

Acylglycerol kinase antibody detects Acylglycerol kinase protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Acylglycerol kinase antibody (GTX107413) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107413 IHC-P Image

Acylglycerol kinase antibody detects Acylglycerol kinase protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Acylglycerol kinase antibody (GTX107413) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107413 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Acylglycerol kinase(GTX107413) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107413 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:H1299
10% SDS PAGE
GTX107413 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107413 WB Image

Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody (GTX107413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107413 WB Image

Acylglycerol kinase antibody detects Acylglycerol kinase protein by western blot analysis.
A. 50 μg mouse heart lysate/extract
10% SDS-PAGE
Acylglycerol kinase antibody (GTX107413) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107413 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody (GTX107413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107413 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using Acylglycerol kinase(GTX107413) antibody at 1:500 dilution.