| Catalog Number | GTX110558 |
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| Product Name | APE1 antibody |
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| Full Name | APEX nuclease (multifunctional DNA repair enzyme) 1 |
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| Synonyms | APEX1|APE|APE1|APEN|APEX|APX|HAP1|REF1|APEX nuclease (multifunctional DNA repair enzyme) 1 |
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| Product Description | Rabbit polyclonal antibody to APE1 (APEX nuclease (multifunctional DNA repair enzyme) 1) |
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| Background | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq] |
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| Host | Rabbit |
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| Clonality | Polyclonal |
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| Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 288 of APE1 |
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| Antigen Species | Human |
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| Species Cross Reactivity | human - Not yet tested in other species |
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| Predicted Cross Reactivity Species | mouse(94%), rat(93%), cow(93%), dogs(95%), pig(94%) |
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| Tested Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
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| Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000,IHC-P: 1:100-1:500,IF:1:100-1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
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| Positive Controls | 293T | A431 | H1299 | HeLa | HepG2 | Molt-4 | Raji |
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| Target | APE1 |
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| Predicted Target Size(KDa) | 36 |
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| Cellular Localization | Nucleus |
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| Form Supplied | Liquid |
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| Concentration | 1 mg/ml |
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| Purification | Purified by antigen-affinity chromatography. |
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| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
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| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
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| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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