| Catalog Number | GTX101074 |
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| Product Name | AKT1 antibody |
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| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
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| Synonyms | AKT1|AKT|MGC99656|PKB|PKB-ALPHA|PRKBA|RAC|RAC-ALPHA|v-akt murine thymoma viral oncogene homolog 1 |
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| Product Description | Rabbit Polyclonal AKT1 antibody ( v-akt murine thymoma viral oncogene homolog 1) |
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| Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
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| Host | Rabbit |
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| Clonality | Polyclonal |
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| Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 266 and 476 of AKT1 |
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| Antigen Species | Human |
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| Species Cross Reactivity | human - Not yet tested in other species |
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| Predicted Cross Reactivity Species | mouse(98%), rat(86%), cow(97%), dogs(83%), pig(98%), zebra fish(83%) |
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| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
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| Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
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| Positive Controls | HeLa_HA-AKT |
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| Predicted Target Size(KDa) | 56 |
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| Cellular Localization | 0 |
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| Form Supplied | Liquid |
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| Concentration | 0.17 mg/ml |
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| Purification | Purified by antigen-affinity chromatography. |
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| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
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| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
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| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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