| Catalog Number | GTX101430 |
| Product Name | ACHE antibody |
| Full Name | acetylcholinesterase (Yt blood group) |
| Product Description | Rabbit polyclonal antibody to ACHE (acetylcholinesterase (Yt blood group)) |
| Synonyms | ARACHE | N-ACHE | YT |
| Background | Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq] |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 43 and 232 of Human ACHE |
| Antigen Species | Human |
| Tested Applications | WB. The usefulness of this product in other applications has not been determined. |
| Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Specificity | This antibody is specific to the Human ACHE protein. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | HepG2 | Molt-4 | Raji |
| Target | ACHE |
| Predicted Target Size(KDa) | 67.81 |
| Cellular Localization | Cell junction | synapse | Secreted | Cell membrane; Peripheral membrane protein | Isoform T: Nucleus | Isoform H: Cell membrane; Lipid-anchor | GPI-anchor; Extracellular side | Cell junction | synapse | Secreted | Cell membrane | Isoform T: Nucleus | Isof |
| Form Supplied | Liquid |
| Concentration | 0.78 mg/ml |
| Purification | Purified by antigen-affinity chromatography. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |