| Catalog Number | GTX111123 |
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| Product Name | SMAD3 antibody |
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| Full Name | SMAD family member 3 |
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| Synonyms | SMAD3|DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396|SMAD family member 3 |
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| Product Description | Rabbit polyclonal antibody to SMAD3 (SMAD family member 3) |
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| Background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq] |
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| Host | Rabbit |
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| Clonality | Polyclonal |
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| Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 122 and 365 of SMAD3 |
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| Antigen Species | Human |
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| Species Cross Reactivity | human - Not yet tested in other species |
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| Predicted Cross Reactivity Species | mouse(89%), rat(89%), cow(89%), zebra fish(96%) |
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| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
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| Application Note | Suggested starting dilutions are as follows. WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
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| Positive Controls | A549 | HeLa | HepG2 | HCT116 |
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| Target | SMAD3 |
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| Predicted Target Size(KDa) | 48 |
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| Post-translation modification | Phosphoprotein |
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| Cellular Localization | Cytoplasm (By similarity) | Nucleus (By similarity) |
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| Form Supplied | Liquid |
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| Concentration | 0.78 mg/ml |
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| Purification | Purified by antigen-affinity chromatography. |
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| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
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| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
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| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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