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Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (GTX70103) by western blot or immunoprecipitation.

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Detection of ATM (Anti-ATM 2C1, GTX70103) or ATR (Anti-ATR 2B5,GTX70109) by western blot in human Raji whole cell extract.
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Product Information

Product NameCat NoPackagePriceSpecial Price
ATM [2C1] antibodyGTX70103100 μgUSD $289.00 -
Qty   Add to CartWish List
Catalog NumberGTX70103
Product NameATM [2C1] antibody
Product DescriptionMouse monoclonal [2C1] to ATM
Synonyms472 | ATM | 114480 | Q13315 | AT1 | ATA | ATC | ATD | ATDC | ATE | DKFZp781A0353 | MGC74674 | TEL1 |
BackgroundATM-2C1 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.
ClonalityMonoclonal
Clone No2C1
HostMouse
IsotypeIgG1
ImmunogenRecombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
Antigen SpeciesHuman
Tested ApplicationsImmunofluorescence, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.
Application NoteFor WB: Use at a dilution of 1:500-1:1,000. Predicted 350 kDa. For IP: Use at a concentration of 1-10 µg/ml. Immunohistochemistry analyses was performed of the cerebral cortex of E14.5 wild-type (+/+) and scid mice - (MC Vemuri, 2001) For immunofluorescence please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Species Cross ReactivityHuman, Rat, Mouse, and Monkey - Not yet tested in other species
Positive ControlsRaji and lymphoblastoid nuclear lysate
TargetATM
Predicted Target Size(KDa)370
Cellular LocalizationNucleus
Form SuppliedLiquid
Concentration1 mg/ml
PurificationProtein G affinity purified
Purification NoteFrom mouse ascitic fluid
Storage BufferPhosphate-buffered saline, pH 7.4, containing no preservatives
Storage InstructionKeep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

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2.Suzuki, K., Kodama, S. and Watanabe, M. J Biol Chem 274: 25571-25575.

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23.Chen, G. and Lee, E.Y-H.P. The product of the ATM gene is a 370-kDa nuclear phosphoprotein. J Biol Chem 271: 33693-33697.
24.Wu X, Shell SM, Liu Y, Zou Y. ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation. Feb 1;26(5):757-64.

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