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Product Name	Cat No	Package	Price	Special Price
ATM [2C1] antibody	GTX70103	100 μg	USD $289.00	-
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Catalog Number	GTX70103
Product Name	ATM [2C1] antibody
Product Description	Mouse monoclonal [2C1] to ATM
Synonyms	472 \| ATM \| 114480 \| Q13315 \| AT1 \| ATA \| ATC \| ATD \| ATDC \| ATE \| DKFZp781A0353 \| MGC74674 \| TEL1 \|
Background	ATM-2C1 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.
Clonality	Monoclonal
Clone No	2C1
Host	Mouse
Isotype	IgG1
Immunogen	Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
Antigen Species	Human
Tested Applications	Immunofluorescence, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.
Application Note	For WB: Use at a dilution of 1:500-1:1,000. Predicted 350 kDa. For IP: Use at a concentration of 1-10 µg/ml. Immunohistochemistry analyses was performed of the cerebral cortex of E14.5 wild-type (+/+) and scid mice - (MC Vemuri, 2001) For immunofluorescence please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Species Cross Reactivity	Human, Rat, Mouse, and Monkey - Not yet tested in other species
Positive Controls	Raji and lymphoblastoid nuclear lysate
Target	ATM
Predicted Target Size(KDa)	370
Cellular Localization	Nucleus
Form Supplied	Liquid
Concentration	1 mg/ml
Purification	Protein G affinity purified
Purification Note	From mouse ascitic fluid
Storage Buffer	Phosphate-buffered saline, pH 7.4, containing no preservatives
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

General References

1.	Chen, G. and Lee, E.Y-H.P. The product of the ATM gene is a 370-kDa nuclear phosphoprotein. J Biol Chem 271: 33693-33697.
2.	Suzuki, K., Kodama, S. and Watanabe, M. J Biol Chem 274: 25571-25575.

Specific References

1.	Sergei Kozlov, Nuri Gueven, Katherine Keating, Jonathan Ramsay, and Martin F. Lavin. J. Biol. Chem., Mar 2003; 278: 9309 - 9317.
2.	Eric J. Perkins, Ayyappan Nair, Dale O. Cowley, Terry Van Dyke, Yung Chang, and Dale A. Ramsden. Genes & Dev., Jan 2002; 16: 159 - 164.
3.	Harry Scherthan, Martin Jerratsch, Sonu Dhar, Y. Alan Wang, Stephen P. Goff, and Tej K. Pandita. Meiotic telomere distribution and Sertoli cell nuclear architecture are altered in Atm- and Atm-p53-deficient mice. Mol. Cell. Biol., Oct 2000; 20: 7773 - 7783.
4.	M C Vemuri, E Schiller, J R Naegele. Cell Death and Differentiation 8, 245 - 255 (22 Mar 2001)
5.	Shang Li, Nicholas S. Y. Ting, Lei Zheng, Phang-Lang Chen, Yael Ziv, Yosef Shiloh, Eva Y.-H. P. Lee, Wen-Hwa Lee. Nature 406, 210 - 215 (13 Jul 2000)
6.	Heather Beamish, Padmini Kedar, Hideo Kaneko, Philip Chen, Toshiyuki Fukao, Cheng Peng, Sergei Beresten, Nuri Gueven, David Purdie, Susan Lees-Miller, Nathan Ellis, Naomi Kondo, and Martin F. Lavin. J. Biol. Chem., Aug 2002; 277: 30515 - 30523.
7.	Baocheng Hu, Xiang-Yang Zhou, Xiang Wang, Zhao-Chong Zeng, George Iliakis, and Ya Wang. J. Biol. Chem., May 2001; 276: 17693 - 17698.
8.	Gang Chen and Eva Y.-H. P. Lee. J. Biol. Chem., Dec 1996; 271: 33693 - 33697.
9.	Jong-Soo Kim, Tatiana B. Krasieva, Vickie LaMorte, A. Malcolm R. Taylor, and Kyoko Yokomori. J. Biol. Chem., Nov 2002; 277: 45149 - 45153.
10.	A. Fedier, M. Schlamminger, V. A. Schwarz, U. Haller, S. B. Howell, and D. Fink. Ann. Onc., Jun 2003; 14: 938 - 945.
11.	Shaun P. Scott, Regina Bendix, Philip Chen, Raymond Clark, Thilo Dörk, and Martin F. Lavin . PNAS, Jan 2002; 99: 925 - 930.
12.	Thilo Dörk, Regina Bendix, Michael Bremer, Dirk Rades, Karin Klöpper, Marion Nicke, Britta Skawran, Ariadne Hector, Paria Yamini, Diana Steinmann, Sabine Weise, Manfred Stuhrmann, and Johann H. Karstens. Cancer Res., Oct 2001; 61: 7608 - 7615.
13.	Lubomir B. Smilenov, David J. Brenner, and Eric J. Hall . Cancer Res., Aug 2001; 61: 5710 - 5713.
14.	Paul R. Borghesani, Frederick W. Alt, Andrea Bottaro, Laurie Davidson, Saime Aksoy, Gary A. Rathbun, Thomas M. Roberts, Wojciech Swat, Rosalind A. Segal, and Yansong Gu . PNAS, Mar 2000; 97: 3336 - 3341.
15.	Yi Wang, David Cortez, Parvin Yazdi, Norma Neff, Stephen J. Elledge, and Jun Qin. Genes & Dev., Apr 2000; 14: 927 - 939.
16.	Ryoko Araki, Ryutaro Fukumura, Akira Fujimori, Yoichi Taya, Yosef Shiloh, Akihiro Kurimasa, Sandeep Burma, Gloria C. Li, David J. Chen, Koki Sato, Yuko Hoki, Kouichi Tatsumi, and Masumi Abe. Cancer Res., Aug 1999; 59: 3543 - 3546.
17.	Michael M Weil, Frances S Kittrell, Yongjia Yu, Maureen McCarthy, Ryan C Zabriskie, Robert L Ullrich. Oncogene 20, 4409 - 4411 (17 Jul 2001)
18.	M L Cortés, C J Bakkenist, M V Di Maria, M B Kastan, X O Breakefield. Gene Therapy 10, 1321 - 1327 (01 Aug 2003)
19.	Pierre-Marie Girard, Enriqueta Riballo, Adrian C Begg, Alastair Waugh, Penny A Jeggo. Oncogene 21, 4191 - 4199 (20 Jun 2002)
20.	Ayumi Kudoh, Masatoshi Fujita, Lumin Zhang, Noriko Shirata, Tohru Daikoku, Yutaka Sugaya, Hiroki Isomura, Yukihiro Nishiyama, and Tatsuya Tsurumi. February 2005, 10.1074/jbc.M411405200
21.	Jia Li and David F. Stern. J. Biol. Chem. published 24 January 2005, 10.1074/jbc.M412445200
22.	Yiyong Liu, Antonio Rusinol, Michael Sinensky, Youjie Wang, Yue Zou. DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A. J Cell Science. Nov. 15;119(Pt 22):4644-9.
23.	Chen, G. and Lee, E.Y-H.P. The product of the ATM gene is a 370-kDa nuclear phosphoprotein. J Biol Chem 271: 33693-33697.
24.	Wu X, Shell SM, Liu Y, Zou Y. ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation. Feb 1;26(5):757-64.

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