| |
|
|
|
|
Hypoxia in tumors is closely associated with tumor aggressiveness and resistance to radio- and chemotherapeutic treatment. Therefore, reliable markers for hypoxia represent both valuable diagnostic markers and potential targets for investigation. When oxygen becomes limited, prolyl hydroxylase is inhibited which leads to HIF-α accumulation and translocation to the nucleus. HIF-α then dimerizes with HIF-β and interacts with cofactors such as p300/CBP and the Pol II complex to activate the hypoxia-response elements (HRE), which activate transcription of various genes including VEGFA, GLUT1, and CA9. |
| |
| |
|
Featured Antibodies |
| |
|
HIF1 alpha antibody
| Cat. No. |
Host & Clonality |
Reactivity |
Application |
|
GTX127309 |
Rabbit Polyclonal |
Hu, Mu |
ICC/IF, IP, WB | |
| |
 |
|
 |
|
 |
|
 |
| |
|
|
|
|
|
|
| Confocal IF analysis of HeLa cells, untreated (left) or CoCl2-treated (right), using HIF-1α (green) and tubulin (red) antibodies. |
|
WB analysis of induced-HIF-1α expression in HCT116 cells. |
|
IP analysis of HIF-1α protein in HIF-1α- overexpressed cells. The precipitated HIF-1α was detected by the same HIF-1α antibody. | |
| |
Temperature Stability
|
 |
|
 |
|
 |
|
 |
| |
|
|
|
|
|
|
| -20 ℃ |
|
1 week at 4 ℃ |
|
1 week at RT |
|
1 week at 37 ℃ |
|
| |
| |
Freeze and Thaw: -20℃ to Room Temperature
|
 |
|
 |
|
Please note that this stability test is for reference purposes only. GeneTex recommends following the specific storage directions listed on each datasheet and storing as small aliquots to prevent repeated freeze/thaw cycles and contamination. |
| |
|
|
|
|
|
|
| Original |
|
3 Freeze and thaws: 1 per 24 hours |
|
|
|
|
|
| |
| |
|
VHL antibody
| Cat. No. |
Host & Clonality |
Reactivity |
Application |
|
GTX101087 |
Rabbit Polyclonal |
Hu |
IHC-P, WB | |
| |
 |
|
 |
|
|
|
|
| |
|
|
|
|
|
|
| Detection of VHL in paraffin-embedded colon. |
|
Detection of VHL in 30 ug of Molt4 whole? cell lysate. |
|
|
|
| |
| |
| |
|
CBP antibody [C3], C-term
| Cat. No. |
Host & Clonality |
Reactivity |
Application |
|
GTX101249 |
Rabbit Polyclonal |
Hu |
ICC/IF, WB | |
| |
 |
|
 |
|
 |
|
|
| |
|
|
|
|
|
|
| Detection of CBP in paraformyldehyde-fixed HeLa cells. |
|
Detection of CBP in 30 ug of H1299 whole? cell lysate. |
|
| |
| |
| |
|
More Hypoxia-Related Products... |
| |