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1 .GTX128101 Wnt3a antibody

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Wnt3a antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman
  • Publication  ( 1 )
Cat No: GTX128101
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Wnt3a antibody used in Western Blot (WB). GTX128101
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  • Datasheet
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    Reference (1)
  • Bio-info
  • Protocol & MSDS
  • Application Information: Wnt3a antibody

Catalog Number GTX128101
☆☆☆☆☆
( 0 )
Publication ( 1 )
Product Name Wnt3a antibody
See all Wnt3a products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)Assay-dependent dilution Y
Western Blot (WB)1:1000-1:10000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size39 kDa (note)
Positive Controlshuman WNT3A-transfected 293T cells
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Wnt3a antibody

Full Namewingless-type MMTV integration site family, member 3A
BackgroundThe WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq]
SynonymsMGC119418 antibody, MGC119419 antibody, MGC119420 antibody, WNT3A antibody, protein Wnt-3a antibody, wingless-type MMTV integration site family, member 3A antibody
Cellular LocalizationSecreted , extracellular space , extracellular matrix
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Wnt3a. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Zebrafish, Xenopus laevis, Chicken
Predict Reactivity NoteMouse (98%), Rat (98%), Zebrafish (87%), Xenopus laevis (86%), Chicken (87%)
ConjugationUnconjugated

  • Storage Conditions: Wnt3a antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Neuroscience > Signaling > Hedgehog signaling
  • Signal Transduction > Signaling through proteolysis > Hedgehog
  • Stem Cell Development > Development > Morphogenesis
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Wnt3a antibody validated data
  • Anti-Wnt3a antibody used in Western Blot (WB). GTX128101
    • GTX128101 WB Image
      Wnt3A antibody detects WNT3A protein by Western blot analysis.
      A. 30 µg 293T whole cell lysate/extract
      B. 30 µg whole cell lysate/extract of human WNT3A-transfected 293T cells
      10 % SDS-PAGE
      Wnt3A antibody (GTX128101) dilution: 1:5000
      Top
  • Anti-Wnt3a antibody used in Western Blot (WB). GTX128101
    • GTX128101 WB Image
      Wnt3A antibody detects WNT3A protein by Western blot analysis.
      A. 30 µg 293T whole cell lysate/extract
      B. 30 µg whole cell lysate/extract of human WNT3A-transfected 293T cells
      10 % SDS-PAGE
      Wnt3A antibody (GTX128101) dilution: 1:5000
      Top
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  • Reference for Wnt3a antibody
Application References Application Reactivity
Ng M et al. A Genome-wide Association Study of Dupuytren Disease Reveals 17 Additional Variants Implicated in Fibrosis. Am J Hum Genet 101 (3):417-427 (2017). IHC-P Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensWNT3A89780P56704606359NM_033131NP_14912239.37

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusWnt3a22416P27467NM_009522NP_03354839.26
Rattus norvegicusWnt3a303181NM_001107005NP_00110047540.09
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.