ZCCHC11 / PAPD3 antibody, C-term
|Application Note||Approx 250-300 kDa and 30-35 kDa band seen in Raji Lysate. Recommended for use at 1-3 µg/ml. Please note that the band we consistently detect in western blots differs from the predicted size of 181kDa. Currently we cannot find an explanation for this in the literature. However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. This antibody is expected to recognize all 3 reported isoforms (as represented by NP_001009881.1; NP_056084.1; NP_001009882.1)|
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX94119-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||zinc finger, CCHC domain containing 11|
|Product Description||Goat polyclonal antibody to ZCCHC11 / PAPD3|
|Background||Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. |
|Synonyms||ZCCHC11, KIAA0191, ZCCHC11 / PAPD3, zinc finger, CCHC domain containing11, PAPD3, PAP associated domain containing3, PAP associated domain containing 3, zinc finger, CCHC domain containing 11, DKFZp779C1943, FLJ42878, TUT4|
|Cellular Localization||Translocates into the cytoplasm following treatment of the cell with LPS. |
|Specificity||This antibody is specific for ZCCHC11 / PAPD3.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Dog, Bovine|