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ZNF189 antibody

Anti-ZNF189 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117129
Anti-ZNF189 antibody used in Western Blot (WB). GTX117129

Cat. No. GTX117129

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ChIP-seq

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZNF189 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IP Assay dependent
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

Jurkat , Raji , K562

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZNF189. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172589

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 189

Cellular Localization

Nucleus

Background

Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ZNF189 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117129

GTX117129 ICC/IF Image

ZNF189 antibody detects ZNF189 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF189 protein stained by ZNF189 antibody (GTX117129) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-ZNF189 antibody used in Western Blot (WB). GTX117129

GTX117129 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
7.5% SDS PAGE
GTX117129 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169