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1 .GTX108592 ZO-1 antibody

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ZO-1 antibody

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse
  • Publication  ( 3 )
Cat No: GTX108592
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
  • Anti-ZO-1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX108592
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
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  • Datasheet
  • Star Review (0)
    Reference (3)
  • Bio-info
  • Protocol & MSDS
  • Application Information: ZO-1 antibody

Catalog Number GTX108592
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( 0 )
Publication ( 3 )
Product Name ZO-1 antibody
See all ZO1 products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Western Blot (WB) : The use of GTX108592 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX108613
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size195 kDa (note) IsoForm (5)
Positive Controls293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116
Form SuppliedLiquid
Concentration0.24 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: ZO-1 antibody

Full Nametight junction protein 1 (zona occludens 1)
BackgroundThis gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
SynonymsDKFZp686M05161 antibody, MGC133289 antibody, ZO-1 antibody, TJP1 antibody, zona occludens protein 1 antibody, zona occludens 1 antibody, tight junction protein ZO-1 antibody, zonula occludens 1 protein antibody, zonula occludens protein 1 antibody, tight junction protein 1 (zona occludens 1) antibody
Cellular LocalizationCell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Dog
Predict Reactivity NoteRat (99%), Dog (100%)
ConjugationUnconjugated

  • Storage Conditions: ZO-1 antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ZO-1 antibody validated data
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
    • GTX108592 WB Image
      Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
    • GTX108592 WB Image
      ZO-1 antibody detects ZO-1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-ZO-1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX108592
    • GTX108592 IHC-P Image
      ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse cervix.
      ZO-1 antibody (GTX108592) diluted at 1:500.
      Top
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
    • GTX108592 WB Image
      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.
      Top
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
    • GTX108592 WB Image
      Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
    • GTX108592 WB Image
      ZO-1 antibody detects ZO-1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-ZO-1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX108592
    • GTX108592 IHC-P Image
      ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse cervix.
      ZO-1 antibody (GTX108592) diluted at 1:500.
      Top
  • Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
    • GTX108592 WB Image
      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.
      Top
Back to Top
  • Reference for ZO-1 antibody
Application References Application Reactivity
Li W et al. The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. J Clin Invest (Epub ahead of print) (2017). WB Human
Jo H et al. Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2?cells. Food Chem Toxicol 108 (Pt A):203-213 (2017). WB Human
Hwang D et al. Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells. Biomed Pharmacother (2016). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensTJP17082Q07157601009NP_001287954205.11
Homo sapiensTJP17082Q07157601009NP_001287955189.71
Homo sapiensTJP17082Q07157601009NP_001317168197.49
Homo sapiensTJP17082Q07157601009NM_003257NP_003248195.49
Homo sapiensTJP17082Q07157601009NM_175610NP_783297187

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusTjp121872P39447NM_009386NP_033412194.78
Mus musculusTjp121872P39447NM_001163574NP_001157046188.89
Rattus norvegicusTjp1292994NM_001106266NP_001099736194.06
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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