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Catalog Number
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GTX108592
(
1
)
|
|
Product Name
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ZO-1 antibody
|
| Applications | IHC-P, WB |
| Application Note |
| Suggested dilution | Reference |
| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
|
| Western blot | 1:500-1:3000* |
Y |
Western blot : The use of GTX108592 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX108613
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Predicted Target Size | 195 kDa (note)
 (5)
|
| Positive Controls | 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 |
| Form Supplied | Liquid |
| Concentration | 0.24
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | tight junction protein 1 (zona occludens 1) |
| Product Description | Rabbit Polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1)) |
| Background | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Synonyms | DKFZp686M05161 antibody, MGC133289 antibody, ZO-1 antibody, TJP1 antibody, zona occludens protein 1 antibody, zona occludens 1 antibody, tight junction protein ZO-1 antibody, zonula occludens 1 protein antibody, zonula occludens protein 1 antibody, tight junction protein 1 (zona occludens 1) antibody |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Rat, Dog |
| Predict Reactivity Note | Rat (99%), Dog (100%) |
| Conjugation | Unconjugated |