ZO-1 antibody [N1N2], N-term
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||195 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , mouse testis|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||tight junction protein 1 (zona occludens 1)|
|Product Description||Rabbit Polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1))|
|Background||This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]|
|Synonyms||DKFZp686M05161 antibody, MGC133289 antibody, ZO-1 antibody, TJP1 antibody, zona occludens protein 1 antibody, zona occludens 1 antibody, tight junction protein ZO-1 antibody, zonula occludens 1 protein antibody, zonula occludens protein 1 antibody, tight junction protein 1 (zona occludens 1) antibody|
|Cellular Localization||Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rat (94%), Dog (95%)|