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Catalog Number
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GTX112477
(
0
)
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Product Name
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alanyl-tRNA synthetase antibody [N3C2], Internal
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| Applications | IHC-P, WB |
| Application Note |
| Suggested dilution | Reference |
| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
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| Western Blot (WB) | 1:500-1:3000* |
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Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Predicted Target Size | 107 kDa (note)
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| Positive Controls | A431 , H1299 , HeLa , HepG2 , mouse liver |
| Form Supplied | Liquid |
| Concentration | 0.23
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | alanyl-tRNA synthetase |
| Background | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq] |
| Synonyms | CMT2N antibody, AARS antibody, alanine tRNA ligase 1, cytoplasmic antibody, alanine--tRNA ligase antibody, renal carcinoma antigen NY-REN-42 antibody, alaRS antibody, alanyl-tRNA synthetase, cytoplasmic antibody, alanyl-tRNA synthetase antibody |
| Cellular Localization | Cytoplasm |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alanyl-tRNA synthetase. The exact sequence is proprietary. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Rat, Xenopus laevis, Chicken, Bovine |
| Predict Reactivity Note | Rat (92%), Xenopus laevis (84%), Chicken (86%), Bovine (92%) |
| Conjugation | Unconjugated |