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  • alanyl-tRNA synthetase antibody [N3C2], Internal

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1 .GTX112477 alanyl-tRNA synthetase antibody [N3C2], Internal

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alanyl-tRNA synthetase antibody [N3C2], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse
Cat No: GTX112477
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX112477
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
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  • Application Information: alanyl-tRNA synthetase antibody [N3C2], Internal

Catalog Number GTX112477
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Product Name alanyl-tRNA synthetase antibody [N3C2], Internal
See all alanyl-tRNA synthetase products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size107 kDa (note)
Positive ControlsA431 , H1299 , HeLa , HepG2 , mouse liver
Form SuppliedLiquid
Concentration0.23 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: alanyl-tRNA synthetase antibody [N3C2], Internal

Full Namealanyl-tRNA synthetase
BackgroundThe human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]
SynonymsCMT2N antibody, AARS antibody, alanine tRNA ligase 1, cytoplasmic antibody, alanine--tRNA ligase antibody, renal carcinoma antigen NY-REN-42 antibody, alaRS antibody, alanyl-tRNA synthetase, cytoplasmic antibody, alanyl-tRNA synthetase antibody
Cellular LocalizationCytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human alanyl-tRNA synthetase. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Xenopus laevis, Chicken, Bovine
Predict Reactivity NoteRat (92%), Xenopus laevis (84%), Chicken (86%), Bovine (92%)
ConjugationUnconjugated

  • Storage Conditions: alanyl-tRNA synthetase antibody [N3C2], Internal

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
alanyl-tRNA synthetase antibody [N3C2], Internal validated data
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
    • GTX112477 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431 (GTX27909)
      B: H1299
      C: Hela
      D: Hep G2 (GTX27900)
      7.5% SDS PAGE
      GTX112477 diluted at 1:1000
      Top
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX112477
    • GTX112477 IHC-P Image
      alanyl-tRNA synthetase antibody [N3C2], Internal detects AARS protein at cytosol on human hepatoma by immunohistochemical analysis.
      Sample: Paraffin-embedded hepatoma.
      alanyl-tRNA synthetase antibody [N3C2], Internal (GTX112477) dilution: 1:500.
      Top
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
    • GTX112477 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse liver
      5% SDS PAGE
      GTX112477 diluted at 1:1000
      Top
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
    • GTX112477 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431 (GTX27909)
      B: H1299
      C: Hela
      D: Hep G2 (GTX27900)
      7.5% SDS PAGE
      GTX112477 diluted at 1:1000
      Top
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX112477
    • GTX112477 IHC-P Image
      alanyl-tRNA synthetase antibody [N3C2], Internal detects AARS protein at cytosol on human hepatoma by immunohistochemical analysis.
      Sample: Paraffin-embedded hepatoma.
      alanyl-tRNA synthetase antibody [N3C2], Internal (GTX112477) dilution: 1:500.
      Top
  • Anti-alanyl-tRNA synthetase antibody [N3C2], Internal used in Western Blot (WB). GTX112477
    • GTX112477 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse liver
      5% SDS PAGE
      GTX112477 diluted at 1:1000
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensAARS16P49588601065, 613287NM_001605NP_001596106.83

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusAars234734Q8BGQ7NM_146217NP_666329106.93
Rattus norvegicusAars292023NM_001100517NP_001093987106.81
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.