alpha 1 Catenin antibody [N3C2], Internal
|Applications||ICC/IF, IHC-Fr, IHC-P, WB|
Not tested in other applications.
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||100 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , NIH-3T3 , JC , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||catenin (cadherin-associated protein), alpha 1, 102kDa|
|Product Description||Rabbit Polyclonal antibody to alpha 1 Catenin (catenin alpha-1)|
|Background||Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. |
|Synonyms||CAP102 antibody, FLJ36832 antibody, FLJ52416 antibody, CTNNA1 antibody, "cadherin-associated protein,102kDa antibody", alpha-catenin antibody, alphaE-catenin antibody, catenin alpha-1 antibody, renal carcinoma antigen NY-REN-13 antibody, alpha-E-catenin antibody, alpha E-catenin antibody, "catenin (cadherin-associated protein), alpha 1, 102kDa antibody", alpha catenin antibody, alpha catenin|
|Cellular Localization||Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human alpha 1 Catenin. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat, Zebrafish|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Rabbit, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Xenopus laevis (93%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%)|