alpha Actinin 2 antibody [N2C1], Internal
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||104 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 , Rat heart|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||actinin, alpha 2|
|Product Description||Rabbit Polyclonal antibody to alpha Actinin 2 (actinin, alpha 2)|
|Background||Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]|
|Synonyms||CMD1AA antibody, ACTN2 antibody, F-actin cross-linking protein antibody, alpha-actinin-2 antibody, alpha-actinin skeletal muscle antibody, "actinin, alpha 2 antibody"|
|Cellular Localization||Cytoplasm , myofibril , sarcomere , Z-disk |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Chicken, Bovine|
|Predict Reactivity Note||Pig (99%), Chicken (97%), Bovine (98%)|