APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , A431 , H1299 , Rat heart
Product Note
Based on sequence homology, this antibody may cross react with ACTN1 protein.
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949577
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actinin alpha 2 , CMD1AA , CMH23
Cellular Localization
Cytoplasm , myofibril , sarcomere , Z-disk
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/templates/default/js/lightbox/images/loading.gif "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
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GTX111167 WB Image
Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111167 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111167 diluted at 1:1000
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GTX111167 WB Image
alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX111167) diluted at 1:5000.
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GTX111167 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2(GTX111167) antibody at 1:200 dilution.
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GTX111167 IHC-P Image
alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111167 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX111167 diluted at 1:3000
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GTX111167 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Actinin 2 antibody [N2C1], Internal (GTX111167) at 1:300 dilution.
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GTX111167 WB Image
Sample (10 μg of whole cell lysate)
A: adult zebrafish
7.5% SDS PAGE
GTX111167 diluted at 1:3000
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REFERENCE
There are currently no references for alpha Actinin 2 antibody [N2C1], Internal (GTX111167). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for alpha Actinin 2 antibody [N2C1], Internal (GTX111167). Be the first to share your experience with this product.
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_20220812_WB_M_tissue_22081423_498.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_WB_w_23060500_847.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_20150903_WB_R_heart_w_23060500_546.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_IFA_w_23060500_354.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_20141128_IHC_w_23060500_965.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_WB_M_w_23060500_334.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_IHC_Z_22111423_487.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_WB_Z_22111423_153.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_20220812_WB_M_tissue_22081423_498.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_WB_w_23060500_847.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_20150903_WB_R_heart_w_23060500_546.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_IFA_w_23060500_354.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_20141128_IHC_w_23060500_965.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_WB_M_w_23060500_334.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_IHC_Z_22111423_487.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_WB_Z_22111423_153.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")