alpha Actinin 4 antibody [N2C1], Internal
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||105 kDa (note)
|Positive Controls||A431 , HepG2 , NIH-3T3 , mouse brain , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||actinin, alpha 4|
|Product Description||Rabbit Polyclonal antibody to alpha Actinin 4 (actinin, alpha 4)|
|Background|| Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]|
|Synonyms||ACTININ-4 antibody, DKFZp686K23158 antibody, FSGS antibody, FSGS1 antibody, ACTN4 antibody, focal segmental glomerulosclerosis 1 antibody, F-actin cross-linking protein antibody, non-muscle alpha-actinin 4 antibody, alpha-actinin-4 antibody, actinin alpha4 isoform antibody, "actinin, alpha 4 antibody"|
|Cellular Localization||Nucleus , Cytoplasm|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human alpha Actinin 4. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (89%), Chicken (95%), Rhesus Monkey (100%), Bovine (99%)|