alpha Catenin antibody [N1N3]

Name: alpha Catenin antibody [N1N3]
Brand: GeneTex
SKU: GTX111168
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-alpha Catenin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111168

Anti-alpha Catenin antibody [N1N3] used in Western Blot (WB). GTX111168

Anti-alpha Catenin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111168

Anti-alpha Catenin antibody [N1N3] used in Immunoprecipitation (IP). GTX111168

Cat. No. GTX111168

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX111168 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

Mouse brain , A431 , HeLa , Rat2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950057

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX111168 IHC-P Image

alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Green: alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111168 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
5% SDS PAGE
GTX111168 diluted at 1:500

GTX111168 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
5% SDS PAGE
GTX111168 diluted at 1:1000

GTX111168 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using alpha Catenin(GTX111168) antibody at 1:500 dilution.

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111168 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:500.

GTX111168 IHC-P Image

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111168 IP Image

alpha 1 Catenin antibody [N1N3] immunoprecipitates CTNNA1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CTNNA1 protein by 4 μg alpha 1 Catenin antibody [N1N3] (GTX111168)
5 % SDS-PAGE
The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (GTX111168) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Lai F et al. Cell Biosci 2023; 13 (1) : 71 RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells.

Application : IHC-P

Reactivity : Mouse

Akin R et al. Int J Mol Sci 2019; 20 (24) Cadmium and Lead Decrease Cell-Cell Aggregation and Increase Migration and Invasion in Renca Mouse Renal Cell Carcinoma Cells.

Yuqi Zhang et al. bioRxiv 2019; (Epub) mDia2 formin selectively interacts with catenins and not E-cadherin to regulate Adherens Junction formation.

Application : ICC/IF

Reactivity : Human

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