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alpha Smooth Muscle Actin antibody [4A4]

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in ELISA (ELISA). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in Flow cytometry (FACS). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in Western Blot (WB). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in IHC (Paraffin sections) (IHC-P). GTX60466

Cat. No. GTX60466

Host

Mouse

Clonality

Monoclonal

Clone Name

4A4

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide of human alpha Smooth Muscle Actin.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887970

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , ACTSA

Cellular Localization

Cytoplasm, cytoskeleton

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60466

GTX60466 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60466 alpha Smooth Muscle Actin antibody [4A4].
Green : alpha Smooth Muscle Actin
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-alpha Smooth Muscle Actin antibody [4A4] used in ELISA (ELISA). GTX60466

GTX60466 ELISA Image

ELISA analysis of antigen using GTX60466 alpha Smooth Muscle Actin antibody [4A4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Flow cytometry (FACS). GTX60466

GTX60466 FACS Image

FACS analysis of HeLa cells using GTX60466 alpha Smooth Muscle Actin antibody [4A4].
Green : alpha Smooth Muscle Actin
Purple : negative control

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Western Blot (WB). GTX60466

GTX60466 WB Image

WB analysis of HeLa (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60466 alpha Smooth Muscle Actin antibody [4A4].

Anti-alpha Smooth Muscle Actin antibody [4A4] used in IHC (Paraffin sections) (IHC-P). GTX60466

GTX60466 IHC-P Image

IHC-P analysis of human duodenum tissue (left) and human esophagus tissue (right) using GTX60466 alpha Smooth Muscle Actin antibody [4A4].

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399