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Catalog Number
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GTX629702
(
1
)
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Product Name
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alpha Smooth Muscle Actin antibody [GT445]
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| Applications | ICC/IF, WB |
| Application Note |
| Suggested dilution | Reference |
| Immunocytochemistry/ Immunofluorescence | 1:100-1:1000* |
|
| Western blot | 1:500-1:10000* |
Y |
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Positive Controls | A431 , HeLa , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Rat-2 , drosophila |
| Form Supplied | Liquid |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified by Protein G. |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Product Description | Mouse monoclonal antibody [GT445] to alpha Smooth Muscle Actin |
| Background | The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| Synonyms | ACTA2 Antibody , ACTSA Antibody , MYMY5 Antibody , AAT6 Antibody , alpha SMA antibody , Smactin antibody , alpha SM actin antibody , ASMA antibody , aortic smooth muscle , Alpha-actin-2 antibody, actin aortic smooth muscle antibody , cell growth inhibiting gene 46 protein antibody |
| Cellular Localization | Cytoplasm , cytoskeleton |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Name | GT445 |
| Target | alpha Smooth Muscle Actin |
| Isotype | IgG1 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Smooth Muscle Actin. The exact sequence is proprietary. |
| Species Reactivity | Human, Mouse, Drosophila, Rat |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Zebrafish, Xenopus laevis, Pig, Rabbit, Chicken, Rhesus Monkey, Bovine |
| Predict Reactivity Note | Zebrafish (100%), Xenopus laevis (99%), Pig (100%), Rabbit (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Conjugation | Unconjugated |