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  • alpha Smooth Muscle Actin antibody [GT445]

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1 .GTX629702 alpha Smooth Muscle Actin antibody [GT445]

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alpha Smooth Muscle Actin antibody [GT445]

Knockout/Knockdown

  • Host / ClonalityMouse Monoclonal
  • Clone NameGT445
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse, Drosophila, Rat
  • Publication  ( 4 )
Cat No: GTX629702
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629702
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
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  • Bio-info
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  • Application Information: alpha Smooth Muscle Actin antibody [GT445]

Catalog Number GTX629702
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Publication ( 4 )
Product Name alpha Smooth Muscle Actin antibody [GT445]
See all alpha Smooth Muscle Actin products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000* Y
Western Blot (WB)1:500-1:10000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Positive Controls293T , A431 , HeLa , HepG2 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Rat-2 , drosophila
Form SuppliedLiquid
Concentration0.75 mg/ml (Please refer to the vial label for the specific concentration)
PurificationAffinity purified by Protein G.

  • Specifications: alpha Smooth Muscle Actin antibody [GT445]

Full Nameactin, alpha 2, smooth muscle, aorta
BackgroundThe protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
SynonymsACTA2 Antibody , ACTSA Antibody , MYMY5 Antibody , AAT6 Antibody , alpha SMA antibody , Smactin antibody , alpha SM actin antibody , ASMA antibody , aortic smooth muscle , Alpha-actin-2 antibody, actin aortic smooth muscle antibody , cell growth inhibiting gene 46 protein antibody
Cellular LocalizationCytoplasm , cytoskeleton
HostMouse
ClonalityMonoclonal
Clone NameGT445
IsotypeIgG1
ImmunogenRecombinant protein encompassing a sequence within the center region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Drosophila, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Zebrafish, Xenopus laevis, Pig, Rabbit, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteZebrafish (100%), Xenopus laevis (99%), Pig (100%), Rabbit (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: alpha Smooth Muscle Actin antibody [GT445]

Storage BufferPBS
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Disease Related > Alzheimer's disease
  • Neuroscience > Disorder > Alzheimer's disease
  • Stem Cell Development > Stem cells > Mesenchymal Stem Cells
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alpha Smooth Muscle Actin antibody [GT445] validated data
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629702
    • GTX629702 ICC/IF Image
      alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: alpha Smooth Muscle Actin protein stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
      A. 30 µg Rat-2 whole cell lysate/extract
      10% SDS-PAGE
      alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:5000
      The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
      A. 30 µg drosophila lysate/extract
      10% SDS-PAGE
      alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
      The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
      A. 30 µg C8D30 whole cell lysate/extract
      B. 30 µg NIH-3T3 whole cell lysate/extract
      C. 30 µg BCL-1 whole cell lysate/extract
      D. 30 µg Raw264.7 whole cell lysate/extract
      E. 30 µg C2C12 whole cell lysate/extract
      10% SDS-PAGE
      alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
      The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629702
    • GTX629702 ICC/IF Image
      alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: alpha Smooth Muscle Actin protein stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
      A. 30 µg Rat-2 whole cell lysate/extract
      10% SDS-PAGE
      alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:5000
      The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
      A. 30 µg drosophila lysate/extract
      10% SDS-PAGE
      alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
      The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702
    • GTX629702 WB Image
      alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
      A. 30 µg C8D30 whole cell lysate/extract
      B. 30 µg NIH-3T3 whole cell lysate/extract
      C. 30 µg BCL-1 whole cell lysate/extract
      D. 30 µg Raw264.7 whole cell lysate/extract
      E. 30 µg C2C12 whole cell lysate/extract
      10% SDS-PAGE
      alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
      The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
Back to Top
  • Reference for alpha Smooth Muscle Actin antibody [GT445]
Application References Application Reactivity
Shih YC et al. Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation. Circ Res (Epub ahead of print) (2018). ICC/IF Mouse
Liu MC et al. Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model. PLoS One 12 (5):e0178152 (2017). ICC/IF Human
Davide P et al. Lipid-Induced Hepatocyte-Derived Extracellular Vesicles Regulate Hepatic Stellate Cells via MicroRNA Targeting Peroxisome Proliferator-Activated Receptor γ. Cell Mol Gastroenterol Hepatol 1 (6):646-663 (2015). WB Human
Guerrero F et al. TGF-β prevents phosphate-induced osteogenesis through inhibition of BMP and Wnt/β-catenin pathways. PLoS One 9 (2):e89179 (2014). ICC/IF Rat
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensACTA259B2R8A4, P62736102620, 611788NP_00130778442.01
Homo sapiensACTA259B2R8A4, P62736102620, 611788NM_001141945NP_00113541742.01
Homo sapiensACTA259B2R8A4, P62736102620, 611788NM_001613NP_00160442.01

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusActa211475P62737NM_007392NP_03141842.01
Rattus norvegicusActa281633B0BMT0, P62738NM_031004NP_11226642.01
    *MSDS
    MSDS-PBS.pdf
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