|
Catalog Number
|
GTX21904
(
5
)
|
|
Product Name
|
alpha Synuclein antibody [4B12]
|
| Applications | Dot, ELISA, IHC, IHC-Fr, IHC-P, WB |
| Application Note | Dot: Use at a dilution of 1/100 - 1/10000. ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 16 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form Supplied | Liquid |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification Note | Ammonium sulfate precipitated and dialyzed tissue culture supernatant. |
| Full Name | synuclein, alpha (non A4 component of amyloid precursor) |
| Background | The implication of alpha synuclein in neurodegenerative diseases started after the identification of non A beta component of amyloid (NAC peptide) in Alzheimeris disease (AD) brains.There is now a general group of synucleinopathies which implicates alpha synuclein in Lewy body syndromes, Parkinsonis disease, and Alzheimeris disease. Certain missense mutations in the alpha synuclein gene (A53T, A30P) have been linked to the familial Parkinsonis disease (PD). Alpha synuclein is the major component of Lewy bodies and Lewy neuritis in sporadic PD, dementia with Lewy Bodies and Lewy Body variant of AD. Lewy bodies are composed of truncated and phosphorylated intermediate neurofilament proteins, alpha synuclein, ubiquitin and associated enzymes. The synuclein phosphoproteins (15-20 kD) are small highly conserved proteins in vertebrates. The synuclein family includes alpha and beta synucleins and loosely related gamma synuclein and synoretin.The expression is abundant in neurons and typically localized at presynaptic terminals. Antibodies to alpha synuclein provide a specific method to detect Lewy bodies and associated pathological mechanism of PD and AD. |
| Synonyms | SNCA, PD1, NACP, PARK4, 6622, MGC110988, P37840, 127750, PARK1, alpha Synuclein |
| Cellular Localization | Cytoplasmic and Nuclear. Membrane-bound in dopaminergic neurons. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Name | 4B12 |
| Isotype | IgG1 |
| Immunogen | Recombinant human alpha synuclein produced in E. coli. |
| Antigen Species | Human |
| Species Reactivity | Human |
| Species Does Not React With | Mouse, Rat |
| Conjugation | Unconjugated |