| Catalog Number | GTX70105 |
| Product Name | ATM antibody [3E8]
see all 'ATM' products |
| Full Name | ataxia telangiectasia mutated |
| Synonyms | | ATD | 114480 | ATA | AT1 | ATC | ATE | DKFZp781A0353 | 472 | ATM | TEL1 | ATDC | Q13315 | MGC74674 | |
| Product Description | Mouse monoclonal [3E8] to ATM |
| Background | ATM-3E8 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 3E8 |
| Subclass | IgG1 |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 980-1512. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Monkey, Rat |
| Applications | IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Recommended Starting Dilutions:
For IHC: Use at a concentration of 2-10 µg/ml.
For IP: Use at a concentration of 2-10 µg/ml.
For WB: Use at a concentration of 1-2 µg/ml. Predicted moolecular weight: 350kDa.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Positive Controls | Raji, HeLa, or Akata |
| Target | ATM |
| Predicted Target Size(KDa) | 370 (Note) |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Protein G purified |
| Purification Note | From Ascites |
| Storage Buffer | Phosphate-buffered saline, pH7.4, containing no preservatives |
| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Notes | For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption. |