|Product Name||ATM antibody [3E8]|
see all 'ATM' products
|Full Name||ataxia telangiectasia mutated|
|Synonyms||| ATD | 114480 | ATA | AT1 | ATC | ATE | DKFZp781A0353 | 472 | ATM | TEL1 | ATDC | Q13315 | MGC74674 ||
|Product Description||Mouse monoclonal [3E8] to ATM|
|Background||ATM-3E8 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.|
|Immunogen||Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.|
|Species Reactivity||Human, Mouse, Monkey, Rat|
|Applications||IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.|
|Application Note||Recommended Starting Dilutions:|
For IHC: Use at a concentration of 2-10 µg/ml.
For IP: Use at a concentration of 2-10 µg/ml.
For WB: Use at a concentration of 1-2 µg/ml. Predicted moolecular weight: 350kDa.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||Raji, HeLa, or Akata|
|Predicted Target Size(KDa) ||370 (Note)|
|Purification||Protein G purified|
|Purification Note||From Ascites|
|Storage Buffer||Phosphate-buffered saline, pH7.4, containing no preservatives|
|Storage Instruction||Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles.|
|Notes||For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption.|