beta Catenin antibody [N1N2-2], N-term
|Applications||FACS, ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||85 kDa (note)
|Positive Controls||A549 , H1299 , HCT116 , HeLa , PC-12 , Mouse brain , *human fibroblast , *LX2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||catenin (cadherin-associated protein), beta 1, 88kDa|
|Product Description||Rabbit Polyclonal antibody to beta Catenin (catenin (cadherin-associated protein), beta 1, 88kDa)|
|Background||Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]|
|Synonyms||CTNNB antibody, DKFZp686D02253 antibody, FLJ25606 antibody, FLJ37923 antibody, CTNNB1 antibody, catenin beta-1 antibody, catenin (cadherin-associated protein), beta 1, 88kDa antibody|
|Cellular Localization||Cytoplasm , Nucleus , cytoskeleton |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Sheep, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (95%), Xenopus laevis (97%), Dog (99%), Pig (99%), Chicken (97%), Sheep (99%), Rhesus Monkey (100%), Bovine (99%)|