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  • beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)

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1 .GTX78442 beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)

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beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)


  • Host / ClonalityMouse Monoclonal
  • Clone NameTU-20
  • ApplicationsFACS, ICC/IF, IHC
  • Species reactivityHuman, Mouse, Pig, Rat, Baboon, Carp, Hamster, Mammal, Bovine
  • Publication  ( 2 )
Cat No: GTX78442
Package Price Qty
100 μgUSD 319.00
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  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
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  • Application Information: beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)

Catalog Number GTX78442
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Publication ( 2 )
Product Name beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)
See all beta III Tubulin products
ApplicationsFACS, ICC/IF, IHC
Application NoteFor Immunocytochemistry: Fixed and permeabilized cells. Use at a dilution of 1:40. Optimal dilutions/concentrations should be determined by the researcher
Positive ControlsNeuro2a mouse neuroblastoma cell line
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by precipitation methods

  • Specifications: beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)

BackgroundThe betaIII-tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
SpecificitySequence ESESQGPK (aa 441-448) of human class III beta-tubulin specific for neurones
HostMouse
ClonalityMonoclonal
Clone NameTU-20
IsotypeIgG1
ImmunogenPeptide - (C)441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Species ReactivityHuman, Mouse, Pig, Rat, Baboon, Carp, Hamster, Mammal, Bovine
ConjugationFITC
Conjugation NoteThe purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

  • Storage Conditions: beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)

Storage BufferPhosphate buffered saline containing 15 mM sodium azide, pH 7.4
Storage InstructionKeep as concentrated solution, aliquot and store at 4°C. Do not freeze. Keep protected from prolonged exposure to light. Short-term exposure to room temperature should not affect the quality of the reagent.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) validated data
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
    • GTX78442 ICC/IF Image
      Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (GTX78442; green; 3 μg/ml). Nuclei were stained with DAPI (blue).
      Top
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
    • GTX78442 ICC/IF Image
      Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
      2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (GTX78442; red).
      Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
      Top
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
    • GTX78442 ICC/IF Image
      Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
      2B - Merged image of co-staining with anti-beta-tubulin (GTX27792; green).
      Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
      Top
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
    • GTX78442 ICC/IF Image
      Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (GTX78442; green; 3 μg/ml). Nuclei were stained with DAPI (blue).
      Top
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
    • GTX78442 ICC/IF Image
      Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
      2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (GTX78442; red).
      Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
      Top
  • Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX78442
    • GTX78442 ICC/IF Image
      Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
      2B - Merged image of co-staining with anti-beta-tubulin (GTX27792; green).
      Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
      Top
Back to Top
  • Reference for beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)
Application References Application Reactivity
Park JH et al. Intracranial extraskeletal myxoid chondrosarcoma : case report and literature review. J Korean Neurosurg Soc 52 (3):246-9 (2012). IHC
Jung G et al. Dedifferentiated Extraskeletal Myxoid Chondrosarcoma of the Masticator Space - A Case Report. The Korean Journal of Pathology. 45:S101-105 (2011). IHC Human
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