| Catalog Number | GTX70115 |
| Product Name | BRCA1 antibody [6B4] - ChIP grade
see all 'BRCA1' products |
| Full Name | breast cancer 1, early onset |
| Synonyms | | 113705 | RNF53 | BRCA1 | BRCAI | 672 | PSCP | BRCC1 | P38398 | IRIS | BROVCA1 | PNCA4 | |
| Product Description | Mouse monoclonal [6B4] to BRCA1 |
| Background | BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51. |
| Specificity | This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 6B4 |
| Subclass | IgG1 |
| Immunogen | BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
| Applications | ChIP assay, ICC/IF, Immunohistochemistry, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Suggested starting dilutions are as follows: WB: 1:1000, ICC/IF: 1:400, IHC, IP, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Positive Controls | HBL100 |
| Target | BRCA1 |
| Predicted Target Size(KDa) | 220 (Note) |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Concentration | 1.36 mg/ml |
| Purification | Protein G purified |
| Purification Note | From mouse ascitic fluid |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservative added |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |