c-Myc antibody [5G3]

Name: c-Myc antibody [5G3]
Brand: GeneTex
SKU: GTX84064
Price: 519.00 USD

Anti-c-Myc antibody [5G3] used in IHC (Paraffin sections) (IHC-P). GTX84064

Anti-c-Myc antibody [5G3] used in Western Blot (WB). GTX84064

Anti-c-Myc antibody [5G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84064

Cat. No. GTX84064

Host	Mouse
Clonality	Monoclonal
Clone Name	5G3
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($519)

See all c-Myc products

Datasheet
Document

View product citations for antibody GTX84064 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:50-1:100
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human Myc (NP_002458) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

nucleoplasm,Nucleolus

Background

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Database

Gene ID: 4609 MYC
UniProt: P01106 MYC

Research Area

DATA IMAGES

GTX84064 IHC-P Image

IHC-P analysis of human breast tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 WB Image

WB analysis of various cell lines using GTX84064 c-Myc antibody [5G3].
Loading : 35 ug per lane

GTX84064 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 WB Image

WB analysis of HEK293T cells transfected with c-Myc plasmid (Right) or empty vector (Left) for 48 hrs using GTX84064 c-Myc antibody [5G3].
Loading : 5 ug per lane

GTX84064 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with c-Myc plasmid using GTX84064 c-Myc antibody [5G3].

GTX84064 IHC-P Image

IHC-P analysis of human lung tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84064 IHC-P Image

IHC-P analysis of human colon tissue using GTX84064 c-Myc antibody [5G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

c-Myc antibody [5G3]

Cat. No. GTX84064

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84064 IHC-P Image

GTX84064 WB Image

GTX84064 IHC-P Image

GTX84064 IHC-P Image

GTX84064 WB Image

GTX84064 ICC/IF Image

GTX84064 IHC-P Image

GTX84064 IHC-P Image

GTX84064 IHC-P Image

GTX84064 IHC-P Image

GTX84064 IHC-P Image

GTX84064 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.