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c-Myc antibody [9E10]

Anti-c-Myc antibody [9E10] used in Western Blot and Immunoprecipitation (WB IP). GTX20032
Anti-c-Myc antibody [9E10] used in Western Blot (WB). GTX20032
Anti-c-Myc antibody [9E10] used in Western Blot (WB). GTX20032

Cat. No. GTX20032

Reference 22
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9E10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP, ELISA

Reactivity

 Human
Package
200 μg ($239)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Product Note

This antibody recognizes the sequence EQKLISEEDL

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered PBS pH7

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.

Purification

Protein G purified
From tissue culture supernatant

Purity

>95% (Determined by SDS-PAGE)

Endotoxin

< 0.002 EU/弮g (Determined by LAL assay)

Conjugation

Unconjugated

RRID

AB_369661

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MRTL,MYC,MYCC,bHLHe39,cMyc,vmyc avian myelocytomatosis viral oncogene homolog,cmyc,MYC proto-oncogene, bHLH transcription factor

Cellular Localization

Nucleus,Nucleoplasm,Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody [9E10] used in Western Blot and Immunoprecipitation (WB IP). GTX20032

GTX20032 WB IP Image

The data was published in the journal PLoS One in 2009. PMID: 19536328

Anti-c-Myc antibody [9E10] used in Western Blot (WB). GTX20032

GTX20032 WB Image

The data was published in the journal PLoS One in 2013.PMID: 23620790

Anti-c-Myc antibody [9E10] used in Western Blot (WB). GTX20032

GTX20032 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 28460437

REFERENCE

Application Reference

Roy S et al. NAR Cancer 2023; 5 (3) : zcad047 Large-scale phenogenomic analysis of human cancers uncovers frequent alterations affecting SMC5/6 complex components in breast cancer.
Shun Li et al. Cells 2021; 10 (9) Cauliflower mosaic virus P6 Dysfunctions Histone Deacetylase HD2C to Promote Virus Infection.
Application : WB
Fu SJ et al. Cells 2020; 9 (6) CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.
Application : WB
Reactivity : Human

Clone Reference

Chang YC et al. Nat Commun 2021; 12 (1):4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.
Anand R et al. EMBO J 2019; (Epub) NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
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Submit a Reference

REVIEW

c-Myc antibody [9E10] Cat. No. GTX20032

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-c-Myc antibody [9E10] used in Western Blot (WB). GTX20032
Date : Anonymous submitted on 06-Nov-2015
Score :
Application Tested : WB
Sample : c-myc-tagged fusion protein-transfected Hep3B cells
Amount used : 10μg
Blocking : 5% dry fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Lane Description : Lane1: Hep3B cells, Lane2: c-myc tagged fusion protein in Hep3B cells, Lane3: c-myc tagged fusion protein in Hep3B cells
Submit a Review
Application Reference
Roy S et al. NAR Cancer 2023; 5 (3):zcad047 Large-scale phenogenomic analysis of human cancers uncovers frequent alterations affecting SMC5/6 complex components in breast cancer.
Shun Li et al. Cells 2021; 10 (9) Cauliflower mosaic virus P6 Dysfunctions Histone Deacetylase HD2C to Promote Virus Infection.
Application : WB
Fu SJ et al. Cells 2020; 9 (6) CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.
Application : WB
Reactivity : Human
Chiu SY et al. PLoS One 2019; 14 (3):e0208665 A nonsense mutant of the hepatitis B virus large S protein antagonizes multiple tumor suppressor pathways through c-Jun activation domain-binding protein1.
Application : WB
Thattikota Y et al. Mol Cell 2018; 69 (4):664-676.e5 Cdc48/VCP Promotes Chromosome Morphogenesis by Releasing Condensin from Self-Entrapment in Chromatin.
Application : ICC/IF, IP
Chunman Li et al. Oncotarget 2017; 8 (16):26460-26470 Trichosanthin increases Granzyme B penetration into tumor cells by upregulation of CI-MPR on the cell surface.
Application : WB
Ding X et al. Neurobiol Dis 2016; Fbxl18 targets LRRK2 for proteasomal degradation and attenuates cell toxicity.
Batuello CN et al. Proc Natl Acad Sci U S A 2015; 112 (6):1749-54 Src phosphorylation converts Mdm2 from a ubiquitinating to a neddylating E3 ligase.
Ma M et al. PLoS Pathog 2014; 10 (3):e1004003 Intracellular Theileria annulata Promote Invasive Cell Motility through Kinase Regulation of the Host Actin Cytoskeleton.
Application : IP
Bailey CC et al. J Biol Chem 2013; 288 (45):32184-93 *-induced transmembrane protein 3 is a type ii transmembrane protein.
Application : ICC/IF
Biswas M et al. Exp Cell Res 2013; 319 (13):1922-31 Glycogen synthase kinase 3 regulates expression of nuclear factor-erythroid-2 related transcription factor-1 (Nrf1) and inhibits pro-survival function of Nrf1.
Chiang YJ et al. PLoS One 2013; 8 (4):e61761 CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration.
Application : WB
Ratsima H et al. Proc Natl Acad Sci U S A 2011; 108 (43):E914-23 Independent modulation of the kinase and polo-box activities of Cdc5 protein unravels unique roles in the maintenance of genome stability.
Application : WB
Mabry R et al. MAbs 2010; 2 (1):20-34 A dual-targeting PDGFRbeta/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo.
Ding X et al. PLoS One 2009; 4 (6):e5949 Regulation of LRRK2 stability by the E3 ubiquitin ligase CHIP.
Application : IP
Cagnac O et al. J Biol Chem 2007; 282 (33):24284-93 Identification and characterization of Vnx1p, a novel type of vacuolar monovalent cation/H+ antiporter of Saccharomyces cerevisiae.
Application : WB
Clone Reference
Chang YC et al. Nat Commun 2021; 12 (1):4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.
Anand R et al. EMBO J 2019; (Epub) NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
Emperador-Melero J et al. Nat Commun 2018; 9 (1):3421 Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.
Shen YR et al. PLoS Genet 2017; 13 (3):e1006631 SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.
Li Q et al. J Biol Chem 2015; 290 (35):21553-67 Akt Phosphorylates Wnt Coactivator and Chromatin Effector Pygo2 at Serine 48 to Antagonize Its Ubiquitin/Proteasome-mediated Degradation.
Stengel KF et al. Proc Natl Acad Sci U S A 2012; 109 (14):5399-404 Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion and signaling mechanism that requires cis-trans receptor clustering.
Package List Price ($)
$ 239