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c-Myc antibody

Anti-c-Myc antibody used in Western Blot (WB). GTX30518

Cat. No. GTX30518

Reference 5

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Human
Package
100 μg ($399)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000-1:20,000
ICC/IF 1:100-1:400
IP 1-4μg antibody/mg lysate
ELISA 1:1,000-1:30,000 (Detection) ; 1:100-1:500 (Coating)
IHC Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_385719

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MRTL , MYC , MYCC , bHLHe39 , cMyc , vmyc avian myelocytomatosis viral oncogene homolog , cmyc , MYC proto-oncogene, bHLH transcription factor

Cellular Localization

Nucleus,Nucleoplasm,Nucleolus

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody used in Western Blot (WB). GTX30518

GTX30518 WB Image

WB analysis of 200, 100, and 50ng of E. coli lysate containing tagged fusion protein using GTX30518 c-Myc antibody.
Dilution : 1:25000

REFERENCE

Application Reference

Blackwood M et al. Mol Ther Methods Clin Dev 2024; 32 (1) : 101200 Biodistribution and safety of a single rAAV3B-AAT vector for silencing and replacement of alpha-1 antitrypsin in Cynomolgus macaques.
Caraballo GI et al. J Virol 2022; 96 (12) : e0070422 The Dengue Virus Nonstructural Protein 1 (NS1) Interacts with the Putative Epigenetic Regulator DIDO1 to Promote Flavivirus Replication in Mosquito Cells.
Application : ICC/IF
Viettri M et al. Sci Rep 2021; 11 (1) : 23489 Flavivirus infections induce a Golgi stress response in vertebrate and mosquito cells.
Application : ICC/IF
Tang Q et al. Biores Open Access 2020; 9 (1) : 219-228 Two-Plasmid Packaging System for Recombinant Adeno-Associated Virus.
Application : ELISA
Reactivity : Human
Tien YT et al. Vet J 2015; Downregulation of the KLF4 transcription factor inhibits the proliferation and migration of canine mammary tumor cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399