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c-Myc antibody [N1], N-term

KO/KD Orthogonal
Anti-c-Myc antibody [N1], N-term used in Western Blot (WB). GTX109636
Anti-c-Myc antibody [N1], N-term used in Western Blot (WB). GTX109636

Cat. No. GTX109636

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Product Note

This antibody can detect the endogenous c-Myc, but it is unable to recognize the c-Myc tag.

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human c-Myc. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950966

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody [N1], N-term used in Western Blot (WB). GTX109636

GTX109636 WB Image

Orthogonal

c-Myc antibody [N1], N-term detects c-Myc protein by Western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody [N1], N-term (GTX109636) diluted at 1:1000.

Anti-c-Myc antibody [N1], N-term used in Western Blot (WB). GTX109636

GTX109636 WB Image

KO/KD

Wild-type (WT) and c-Myc knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody [N1], N-term (GTX109636) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ya-Chu Chang et al. Nat Commun 2021; 12 (1) : 4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.
Tsai CH et al. Biochim Biophys Acta 2015; 1852 (5) : 851-61 Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.
Application : WB
Reactivity : Human
Pfaffenbach KT et al. J Cell Physiol 2012; 227 (12) : 695362 GRP78/BiP is a novel downstream target of IGF-1 receptor mediated signaling.
Application : WB
Tsai YS et al. Toxicol Sci 2011; 123 (2) : 480-90 Areca nut induces miR-23a and inhibits repair of DNA double-strand breaks by targeting FANCG.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169