|Background||Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspDETECTTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm. |
‧ Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm).
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Detection of activated caspases in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 30 minutes
‧ Fast and convenient
‧ D2R is more cell-permeable than other fluorometric caspase substrates, activation of caspases can easily be measured in intact cells by flow cytometry.
DTT (1 M)
D?R Incubation Buffer