|Background||Activation of caspases plays a central role in apoptosis. The CaspILLUME? Fluorescein Active Caspase-3 Staining Kit provides a convenient means for sensitive detection of activated caspase-3 in living cells. The assay utilizes the caspase-3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. FITC-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. |
‧ Detection method- Fluorescence microscopy, Flow cytometry and plate reader (Ex. = 485 nm and Em. = 535 nm)
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Sensitive detection of activated caspase-3 in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ The FITC label allows for direct detection of the activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.