caspILLUME Green Active Caspase-9 Staining Kit
|Full Name||Assay for caspase-9 activity in living cells|
|Synonyms||Caspase 9 Activity Assay, Apoptosis Assay, Caspase-9 Activity Assay, Caspase 9 Assay, Caspase-9 Assay, Caspase9 Assay, Caspase9 Activity Assay|
|Product Description||Assay for caspase-9 activity in living cells|
|Background||Activation of caspases plays a central role in apoptosis. The CaspILLUME? Fluorescein Active Caspase-9 Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells. |
‧ Detection method- Fluorescence microscopy, Flow cytometry and plate reader (Ex. = 485 nm and Em. = 535 nm)
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Sensitive detection of activated caspase-9 in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ The FITC label allows for direct detection of the activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
|Storage Instruction||Store at -20°C. Product has an expected shelf life of 6-12 months.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|