|Activation of caspases plays a central role in apoptosis. The CaspILLUMETM Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells. |
‧ Detection method- Fluorescence microscopy, plate reader (Ex/Em = 540/570 nm) and Flow cytometry
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Detection of activated caspases in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 1-2 hours
‧ Fast and convenient
‧ The red fluorescence label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.