Product Name
Cyclin
Catalog Number
GTX26552
Product Description:
Cyclin antibody panel ( anti - Cyclin A B1 D1 E )
Application Note:
Western Blotting (1:500-1:2000 except anti-cyclin E, 1:200-1:500) ELISA (1:500-1:2000 except anti-cycin E, 1:2000) IP. Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry.
Background:
The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted fashion to drive the stages of the cell cycle. Cyclins combine with cyclin dependent kinases (cdks) to form activated kinases that phosphorylate targets leading to cell cycle regulation. A breakdown in the regulation of this cycle can lead to out of control growth and contribute to tumor formation. Defects in many of the molecules that regulate the cell cycle have been implicated in cancer.
Tested Application:
ELISA, IHC (Frozen sections), Western blot
Cross Reactivity:
This kit contains immunological reagents suitable for the analysis of Human, Mouse and Rat cyclins. Cross reactivity with cyclins from other species may also occur.
Storage Instruction:
Store at +4°C short term. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage Buffer:
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Cross-Reactivity:
This kit contains immunological reagents suitable for the analysis of Human, Mouse and Rat cyclins. Cross reactivity with cyclins from other species may also occur.
Target Protein:
Cyclin
Clonality:
Polyclonal
Tested Applications:
ELISA, IHC (Frozen sections), Western blot
Specificity:
Cyclin antibodies recognize only their cognate cyclin.
Immunogen:
Fusion proteins corresponding to the human cyclin A, B1, D1 and E genes.
Host:
Rabbit
Storage:
Store at +4°C short term. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form:
Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG [H&L] (Goat) provided as 2mg lyophilized. Add 1ml to make up to 2.0 mg/ml. Use at 1:7,000 to 1:30,000.
The Kit contains 100 ul each of:
Anti-Cyclin A (GTX22097)
Anti-Cyclin B1 (GTX22096)
Anti-Cyclin D1 (GTX21224)
Anti-Cyclin E (GTX22094)
and 2mg of Peroxidase Conjugated Affinity Purified Goat Anti-Rabbit IgG [H&L] (GTX26721). Reconstitute in 1.0 ml of deionized water.
General References:
1. Koff, A., Cross, F., Fisher, A., Schumaker, J., Leguellec, K., Philippe, M. and Roberts, J. M. (1991) Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell 66; 1217-1228.
1991
2. Koff, A., Giordano, A., Desai, D., Yamashita, K., Harper, J. W., Elledge, S., Nishimoto, T., Morgan, D. O., Franza, B. R. and Roberts, M. (1992) Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science 257; 1689-1694.
1992
3. Claudio, P. P., De Luca, A., Howard, C. M., Baldi, A., Firpo, E. J., Koff, A., Paggi, M. G. and Giordano, A. (1996) Functional analysis of pRb/p130 intereaction with cyclins. Cancer Res. 56; 2003-2008.
1996
4. Xiong, Y., Connolly, T., Futcher, B., and Beach, D. (1991) Human D-type cyclin. Cell 65; 691-699.
1991
5. Pines, J. and Hunter, T. (1990) Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature 346; 760-763.
1990
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