Product Name
NFkB
Catalog Number
GTX26555
Product Description:
NFkB antibody panel with control peptides
Application Note:
Immunoprecipitation immunoblotting (1:1000) ELISAsupershift assays, reactive against p50:p50 homodimers and p:50:p65 heterodimers, all p65 (Rel A) containing human and mouse NFkB, all cRel containing human NFkB complexes using 0.5 to 1.0 ml per assay
Background:
The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the disregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.
Tested Application:
ELISA, Electrophoretic mobility shift assay, Immunoprecipitation, Western blot
Cross Reactivity:
Anti-p50: Human (no reaction is observed against the analogous mouse protein). Anti-NFkB p65 (Rel A): human and mouse. Anti-NFkB cRel: human (no reaction is observed against the anologous Mouse protein).Anti-IkBa: human, mouse and rat.
Purity:
Whole antiserum
Storage Instruction:
Store at 4 °C. Aliquot and store at -20 °C long-term.
Storage Buffer:
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Cellular Localization:
Cytoplasmic and Nuclear
Target Protein:
NFkB
Purification Note:
This product was prepared from monospecific antiserum by delipidation and defibrination.
Tested Applications:
ELISA, Electrophoretic mobility shift assay, Immunoprecipitation, Western blot
Specificity:
Anti-p50: Human NFkB p50 (NFKB1) and its precursor protein.All antibodies may react with non-specific proteins.
Immunogen:
Human NFkB p50 (NFKB1) peptide (region near the N-terminus of the human protein). NFkB p65 (Rel A) peptide (region near the C-terminus of the human protein). NFkB cRel peptide (region near the C-terminus of the human protein).IkBa peptide (region near the C-terminus of the human protein).All conjugated to Keyhole Limpet Hemocyanin (KLH).
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