Product Name
ADAM10 peptide
Catalog Number
GTX27868
Product Description:
ADAM10 ( C - term ) peptide ( human )
Application Note:
The peptide is used for blocking the activity of anti-ADAM10.Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocksthe antibody activity in Western blotting.
Background:
Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, Notch and its ligand Delta are all membrane bound molecules, which are cleaved by proteases. ADAM10, a metalloprotease-disintegrin from the family of mammalian ADAMs (a disintegrin and metalloprotease), cleaves amyloid plaque protein, TNF-alpha, Notch, and its ligand Delta. ADAM10 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. In bovine kidney, ADAM10 cleaves Type IV collagen. ADAM10 is efficiently inhibited by the endogenous MMP inhibitors TIMP1 and TIMP3, but not by TIMP2 and TIMP4. ADAM10 is widely expressed in tissues and can be found on the cell surface and within the cell. The genes encoding human, mouse and bovine ADAM10 are designated ADAM10, kuzbanian (KUZ), and MADM respectively and have 97% sequence identity with each other and with rat ADAM10. There is 45% identity between mouse and Drosophila.
Tested Application:
Blocking
Sequence:
PQRQRPRESYQMGHMRR; Amino acids 732 to 748 of human ADAM10
Purity:
n/a
Storage Instruction:
Store at -20 °C. Stable for one year.
Concentration:
0.2 mg/ml
Storage Buffer:
PBS with 0.1% BSA 0.02% sodium azide pH7.2
Tested Applications:
Blocking
Concentration Unit:
mg/ml
Storage:
Store at -20°C. Stable for one year.
General References:
1. Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumornecrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 1997;272:24588-93.
1997
2. Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumornecrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 1997;272:24588-93.
1997
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