Product Name
Albumin protein (FITC)
Catalog Number
GTX28030
Product Description:
Albumin ( human ) protein ( FITC )
Application Note:
FACS: Use at an assay dependant concentration. IM: Use at an assay dependant concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Serum albumin, a globular unglycosylated protein of molecular weight 65 kDa, is the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted albumin.
Tested Application:
Flow cytometry/FACS, Immunomicroscopy
Conjugation:
FITC
Purity:
n/a
Storage Instruction:
Store vial at 4 °C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Concentration:
1 mg/ml
Storage Buffer:
Preservative: 0.01% Sodium AzideConstituents: 10mg/ml Polyethylene Glycol, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Cellular Localization:
Secreted
Conjugation Note:
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Human Albumin.
Target Protein:
Albumin
Purification Note:
This product was prepared from chromatographically pure Human Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Human Albumin.
Tested Applications:
Flow cytometry/FACS, Immunomicroscopy
Conjugation Type:
FITC
Form:
Lyophilized Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000) Preservative: 0.01% (w/v) Sodium Azide
Concentration Unit:
mg/ml
Storage:
Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
General References:
1. The and Feltkamp, Immunology 18; 865, 1970.
1970
2. The and Feltkamp, Immunology 18; 865, 1970.
1970
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