Product Name
Albumin protein (HRP)
Catalog Number
GTX28032
Product Description:
Albumin ( human ) protein ( HRP )
Application Note:
Dot: Use at an assay dependant concentration. ELISA: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IHC-Fr: Use at an assay dependant concentration. IM: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Serum albumin, a globular unglycosylated protein of molecular weight 65 kDa, is the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted albumin.
Tested Application:
Dot blot, ELISA, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Immunomicroscopy, Western blot
Conjugation:
HRP
Purity:
n/a
Storage Instruction:
Store vial at 4 °C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Concentration:
1 mg/ml
Storage Buffer:
Preservative: 0.01% Gentamicin SulfateConstituents: 10mg/ml Polyethylene Glycol, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Cellular Localization:
Secreted
Conjugation Note:
Horseradish Peroxidase (HRP).
Target Protein:
Albumin
Purification Note:
This product was prepared from chromatographically pure Human Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Albumin.
Tested Applications:
Dot blot, ELISA, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Immunomicroscopy, Western blot
Conjugation Type:
HRP
Form:
Lyophilized
Concentration Unit:
mg/ml
Storage:
Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
General References:
1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
1981
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