Product Name
Albumin protein (Biotin)
Catalog Number
GTX28033
Product Description:
Albumin ( human ) protein ( biotin )
Application Note:
Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
Background:
Serum albumin, a globular unglycosylated protein of molecular weight 65 kDa, is the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted albumin.
Tested Application:
Dot blot, ELISA, Immunomicroscopy, Western blot
Conjugation:
Biotin
Purity:
n/a
Storage Instruction:
Store at +4°C short term. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration:
1 mg/ml
Storage Buffer:
0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2. Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
Cellular Localization:
Secreted
Conjugation Note:
Conjugated to Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Target Protein:
Albumin
Purification Note:
This product was prepared from chromatographically pure Human Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Albumin.
Tested Applications:
Dot blot, ELISA, Immunomicroscopy, Western blot
Conjugation Type:
Biotin
Form:
Lyophilized. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol.
Concentration Unit:
mg/ml
Storage:
Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
General References:
1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
1990
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