Product Name
Acinus peptide
Catalog Number
GTX28367
Product Description:
Acinus ( C - term ) peptide ( human )
Application Note:
The peptide is used for blocking the antibody activity of Acinus (catalog number GTX27352). Itusually blocks the antibody activity completely in Western blot by incubating the peptide withequal volume of antibody for 30 min at 37°C
Background:
Chromatin condensation and nuclear fragmentation (CCNF)is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS.
Tested Application:
Blocking
Sequence:
TRTALHGVKWPQSNPK, Amino acids 1065 to 1080 of human AcinusL, 338 to 353 of human AcinusS', or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (1). The selected antigenic sequence is located near the C-terminus of the cleaved active peptide p17.
Purity:
n/a
Storage Instruction:
Store at -20 °C. Stable for one year.
Concentration:
0.2 mg/ml
Storage Buffer:
PBS with 0.1% BSA 0.02% sodium azide pH7.2
Tested Applications:
Blocking
Specificity:
TRTALHGVKWPQSNPK, Amino acids 1065 to 1080 of human AcinusL, 338 to 353 of human AcinusS', or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (1). The selected antigenic sequence is located near the C-terminus of the cleaved active peptide p17.
Concentration Unit:
mg/ml
Storage:
Store at -20 °C. Stable for one year.
General References:
1. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated proteinrequired for Apoptotic chromatin condensation. Nature 1999 401:168-73.
1999
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